Jung, YJ Youn, JY Ryu, JC Surh, YJ 2024-01-21T12:38:16Z 2024-01-21T12:38:16Z 2021-09-05 2001-03-01 0027-5107 https://pubs.kist.re.kr/handle/201004/140621 Salsolinol (SAL) is a tetrahydroisoquinoline neurotoxin that has been speculated to contribute to pathophysiology of Parkinson's disease and chronic alcoholism. The compound is also found in certain beverages and food stuffs, including soy sauce, beer and bananas. Despite potential human exposure to SAL and its endogenous formation, little is known about the genotoxic or carcinogenic potential of this substance. In the present investigation, SAL induced DNA damage in cultured Chinese hamster lung (CHL) fibroblasts as assessed by single cell gel electrophoresis (Comet). CHL cells treated with SAL also exhibited higher frequencies of chromosomal aberrations than did vehicle-treated controls. Our recent study has revealed that SAL in combination with Cu(II) causes the strand scission in phi X174 supercoiled DNA [Neurosci. Lett. 238 (1997) 95]. In line with this notion, addition of cupric ion potentiated the DNA damaging and clastogenic activity of SAL. Antioxidant vitamins, such as Vitamin C and Vitamin E, and reduced glutathione inhibited clastogenicity of SAL: suggesting the involvement of reactive oxygen species (ROS) in SAL-induced DNA damage and genotoxicity in CHL cells. (C) 2001 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV NEUROBLASTOMA SH-SY5Y CELLS PARKINSONS-DISEASE L-DOPA STRAND SCISSION RAT-BRAIN SUBSTANTIA-NIGRA PC12 CELLS IN-VITRO 6-HYDROXYDOPAMINE NEUROTOXIN Salsolinol, a naturally occurring tetrahydroisoquinoline alkaloid, induces DNA damage and chromosomal aberrations in cultured Chinese hamster lung fibroblast cells Article 10.1016/S0027-5107(00)00156-1 1 MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, v.474, no.1-2, pp.25 - 33 MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 474 1-2 25 33 scie scopus 000167492500003 2-s2.0-0035283237 Biotechnology & Applied Microbiology Genetics & Heredity Toxicology Biotechnology & Applied Microbiology Genetics & Heredity Toxicology Article NEUROBLASTOMA SH-SY5Y CELLS PARKINSONS-DISEASE L-DOPA STRAND SCISSION RAT-BRAIN SUBSTANTIA-NIGRA PC12 CELLS IN-VITRO 6-HYDROXYDOPAMINE NEUROTOXIN salsolinol tetrahydroisoquinolines chromosomal aberrations comet assay Chinese hamster lung fibroblasts redox cycling