Jeong, S Eom, TY Kim, SJ Lee, SW Yu, J 2024-01-21T12:43:06Z 2024-01-21T12:43:06Z 2021-09-05 2001-02-16 0006-291X https://pubs.kist.re.kr/handle/201004/140705 Sialyl Lewis X (sLe(X)) is a tetra-saccharide glycoconjugate of membrane proteins. It acts as a ligand for the selectin proteins during cell adhesion of inflammatory process. Aberrant overexpression of sLeX is also a characteristic of various cancer cells, especially for highly malignant ones. In this paper, the sLe(X)-specific RNA aptamer was selected using a random RNA library and its affinity and specificity were measured by Surface Plasmon Resonance technique. Affinity of the selected RNA was increased about 1000-fold as compared with the original RNA pool. RNA aptamer bound more specifically to its cognate sugar than to any other similar sugars. Inhibition of the cell adhesion was also shown by in vitro static assay of sLe(X)-expressing HL60 cells to the E- and P-selectins. It suggests that the high affinity carbohydrate specific RNA aptamer could be used as an alternative to the antibody. (C) 2001 Academic Press. English ACADEMIC PRESS INC ELSEVIER SCIENCE LECTIN-CARBOHYDRATE INTERACTIONS P-SELECTIN MONOCLONAL-ANTIBODIES LIGANDS RECOGNITION CARCINOMA EVOLUTION LUNG METASTASIS EXPRESSION In vitro selection of the RNA aptamer against the Sialyl Lewis X and its inhibition of the cell adhesion Article 10.1006/bbrc.2001.4327 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.281, no.1, pp.237 - 243 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 281 1 237 243 scie scopus 000167116900036 2-s2.0-0034814741 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article LECTIN-CARBOHYDRATE INTERACTIONS P-SELECTIN MONOCLONAL-ANTIBODIES LIGANDS RECOGNITION CARCINOMA EVOLUTION LUNG METASTASIS EXPRESSION RNA combinatorial library in vitro selection RNA aptamer Sialyl Lewis X E-selectin P-selectin cell adhesion