Choi, MH Chung, BC 2024-01-21T12:45:48Z 2024-01-21T12:45:48Z 2021-09-05 2001-02 0003-2654 https://pubs.kist.re.kr/handle/201004/140754 An improved screening method was developed for 3&apos;-hydroxystanozolol and its 17-epimer in human urine involving gas chromatography-mass spectrometry (GC-MS) with N-isobutyloxycarbonyl (isoBOC) and O-trimethylsilyl (TMS) derivatization. A procedure was reported previously for the pentane extraction of many steroids from urine in doping control, but it was not suitable for the detection of stanozolol metabolites. Compared with the n-pentane extraction method, which gave a poor recovery (< 10%), isoBOC extraction resulted in a good recovery (> 80%). The sensitivity and specificity of mixed N-isoBOC-O-TMS derivatization were adequate for the detection of 3&apos;-hydroxystanozolol and its 17-epimer when 3 ml of urine was used with spiking at a level of 2 ng ml(-1). When applied to a stanozolol-positive urine sample, the proposed method allowed rapid and sensitive screening for the detection of 3&apos;-hydroxystanozolol and its 17-epimer. English ROYAL SOC CHEMISTRY CHROMATOGRAPHY-MASS-SPECTROMETRY ANABOLIC-STEROID METABOLITES URINARY METABOLITES IDENTIFICATION STANOZOLOL HAIR N-Isobutyloxycarbonylation for improved detection of 3 &apos;-hydroxystanozolol and its 17-epimer in doping control Article 10.1039/b007929o 1 ANALYST, v.126, no.3, pp.306 - 309 ANALYST 126 3 306 309 scie scopus 000167227400008 2-s2.0-0035072673 Chemistry, Analytical Chemistry Article CHROMATOGRAPHY-MASS-SPECTROMETRY ANABOLIC-STEROID METABOLITES URINARY METABOLITES IDENTIFICATION STANOZOLOL HAIR N-isobutyloxycarbonylation