Park, SJ Kim, YC Suh, SH Rhim, H Sim, JH Kim, SJ So, I Kim, KW 2024-01-21T13:08:18Z 2024-01-21T13:08:18Z 2022-01-10 2000-12 0021-521X https://pubs.kist.re.kr/handle/201004/140892 The ion channel conductances that regulate the membrane potential was investigated by using a perforated patch-clamp technique in rabbit aorta endothelial cells (RAECs). The whole-cell current/voltage (I-V) relation showed a slight outward rectification under physiological ionic conditions. The resting membrane potential was -23.3+/- 1.1 mV (mean+/-SEM, n=19). The slope conductances at the potentials of -80 and 50mV were 31.0+/-4.0 and 62.8+/-7.1 pS pF(-1), respectively (n=15). Changes in the extracellular and intracellular Cl- concentrations did not affect the reversal potential on I-V curves. The background nonselective cationic (NSC) current was isolated after the K+ current was suppressed. The relative permeabilities calculated from the changes in reversal potentials using the constant-field theory were P-K: P-Cs: P-Na: P-Li = 1:0.87:0.40:0.27 and P-Cs: P-Ca = 1:0.21. Increases in the external Ca2+ decreased the background NSC current in a dose-dependent manner. The concentration for half block by Ca2+ was 1.1+/-0.3 mM (n=7). Through the continuous recording of the membrane potential in a current-clamp mode, it was found that the background NSC conductance is the major determinant of resting membrane potential. Taken together, it could be concluded that the background NSC channels function as the major determinant for the resting membrane potential and can be responsible for the background Ca2+ entry pathway in freshly isolated RAECs. English CENTER ACADEMIC PUBL JAPAN PIG CORONARY-ARTERY POTASSIUM CHANNELS SMOOTH-MUSCLE ION CHANNELS K+ CHANNELS CONDUCTANCE HISTAMINE BLOCK Background nonselective cationic current and the resting membrane potential in rabbit aorta endothelial cells Article 10.2170/jjphysiol.50.635 1 JAPANESE JOURNAL OF PHYSIOLOGY, v.50, no.6, pp.635 - 643 JAPANESE JOURNAL OF PHYSIOLOGY 50 6 635 643 scie scopus 000167502600009 Physiology Physiology Article PIG CORONARY-ARTERY POTASSIUM CHANNELS SMOOTH-MUSCLE ION CHANNELS K+ CHANNELS CONDUCTANCE HISTAMINE BLOCK rabbit aorta endothelial cell background nonselective cationic current resting membrane potential relative permeability