Choi, S Yu, E Lee, YS Yoo, YS 2024-01-21T13:09:48Z 2024-01-21T13:09:48Z 2021-09-04 2000-11-30 1225-8687 https://pubs.kist.re.kr/handle/201004/140919 The nerve growth factor (NGF) induces neuronal differentiation and neurite outgrowth of PC12 cells, whereas epidermal growth factors (EGF) stimulate growth and proliferation of the cells. In spite of this difference, NGF-or EGF-treated PC12 cells share various properties in cellular signaling pathways. These include the activation of the phosphoinositide (PI)-3 kinase, 70 kDa S6 kinase, and in the mitogen-activated protein (MAP) kinase pathway, following the binding of these growth factors to intrinsic receptor tyrosine kinases (RTKs), Therefore, many studies have been attempted to access the critical signaling events in determining the differentiation and proliferation of PC12 cells. In this study, we investigated the cytosolic phospholipase A(2) (cPLA(2)) in neurite behavior in order to identify the differences of signaling pathways between the NGF-induced differentiation and the EGF-induced proliferation of PC12 cells. We have showed here that the cPLA(2) was translocated from cytosol to membrane only in NGF-treated cells. We also demonstrated that this translocation is associated with NGF-induced activation of phospholipase C-gamma (PLC-gamma), which elevates intracellular Ca2+ concentration. These results reveal that the translocation of cPLA(2) may be a requisite event in the neuronal differentiation of PC12 cells. Various phospholipase inhibitors were used to confirm the importance of these enzymes in the differentiation of PC12 cells. Neomycin B, a PLC inhibitor, dramatically inhibited the neurite outgrowth, and two distinct PLA(2) inhibitors, 4-bromophenacyl bromide (BPB) and arachidonyltrifluoro-methyl ketone (AACOCF(3)) also suppressed the neurite outgrowth of the cells, as well. Taken together, these data indicated that cPLA(2) is involved in NGF-induced neuronal differentiation and neurite outgrowth of PC12 cells. English SPRINGER-VERLAG SINGAPORE PTE LTD TYROSINE PHOSPHORYLATION KINASE ACTIVATION FACTOR RECEPTORS MAP KINASE SPECIFICITY INHIBITION EGF IMMUNOREACTIVITY ENHANCEMENT APOPTOSIS Involvement of cytosolic phospholipase A(2) in nerve growth factor-mediated neurite outgrowth of PC12 cells Article 1 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.33, no.6, pp.525 - 530 JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 33 6 525 530 scie scopus 000165575200015 2-s2.0-0005222820 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article TYROSINE PHOSPHORYLATION KINASE ACTIVATION FACTOR RECEPTORS MAP KINASE SPECIFICITY INHIBITION EGF IMMUNOREACTIVITY ENHANCEMENT APOPTOSIS cytosolic phospholipase A(2) differentiation nerve growth factor PC12