Choi, MH Kim, KR Chung, BC 2024-01-21T13:10:11Z 2024-01-21T13:10:11Z 2021-09-05 2000-11-03 0021-9673 https://pubs.kist.re.kr/handle/201004/140926 An efficient method is described for the simultaneous determination of hair polyamines, such as 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine, by gas chromatography-mass spectrometry (GC-MS) with the selected ion-monitoring (SIM) mode. The method is based on the extractive two-phase ethoxycarbonyl (EOC) reaction of amino functions in aqueous solutions combined with subsequent pentafluoropropionyl (PFP) derivatization of the remaining active hydrogen atoms for the direct analysis by GC-SIM-MS. The detection Limits for SIM of the polyamines as N-EOC-N-PFP derivatives ranged from 1 to 10 ng/g hair, while their recovery rates varied in the range of 76.42-93.38%. This method demonstrated a good overall accuracy (% bias) and precision (% C.V.) as 3.32-11.05% and 5.88-14.71%, respectively. When applied to 0.6 M HCl extracts of hair samples from 11 healthy men and 19 healthy women, all five polyamines were positively detected at the concentrations of 8.82-871.87 ng/g. Both in median and mean concentrations, the most abundant hair polyamine was spermidine, followed by spermine, putrescine, 1,3-diaminopropane and cadaverine in the male group, while the order of 1,3-diaminopropane and cadaverine was reversed in the female group. The levels of polyamines, except for cadaverine, in hair specimens studied were found to be higher in men than in women. (C) 2000 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV URINARY POLYAMINE CANCER-PATIENTS BIOLOGICAL-FLUIDS BREAST-CANCER DISEASE AMINES DERIVATIZATION DIAGNOSIS EXCRETION GROWTH Determination of hair polyamines as N-ethoxycarbonyl-N-pentafluoropropionyl derivatives by gas chromatography-mass spectrometry Article 10.1016/S0021-9673(00)00800-1 1 JOURNAL OF CHROMATOGRAPHY A, v.897, no.1-2, pp.295 - 305 JOURNAL OF CHROMATOGRAPHY A 897 1-2 295 305 scie scopus 000165093000025 2-s2.0-0034602467 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article URINARY POLYAMINE CANCER-PATIENTS BIOLOGICAL-FLUIDS BREAST-CANCER DISEASE AMINES DERIVATIZATION DIAGNOSIS EXCRETION GROWTH derivitisation GC hair polyamines