Kim, Y Lee, Y Kim, M Yim, YH Lee, W 2024-01-21T14:38:07Z 2024-01-21T14:38:07Z 2021-09-04 2000-01 0951-4198 https://pubs.kist.re.kr/handle/201004/141713 Gestrinone was studied by HPLC for screening and by GC/MS for confirmation. Three unknown peaks were found by HPLC which are probably the metabolites of gestrinone, and conjugated gestrinone in dosed human urine. The metabolites and gestrinone were excreted as the conjugated forms, The total amounts of metabolite 1 and conjugated gestrinone, recovered after 48 h, were 0.20 and 0.32 mg, respectively. When metabolite 1 was tested by LC/MS and LC/MS/MS, the parent ion was m/z 327, [MH](+), and fragment ions were seen at m/z 309 [MH - H2O](+), 291 [MH - 2H(2)O](+), 283, 263 and 239, The TMS-enol-TMS ether derivative of gestrinone has three peaks in the GC/MS chromatogram formed by tautomerism. The reproducibility of the derivatization method was stable and recoveries were over 87% when spiked into blank urine. Copyright (C) 2000 John Wiley & Sons, Ltd. English JOHN WILEY & SONS LTD Determination and excretion study of gestrinone in human urine by high performance liquid chromatography and gas chromatography/mass spectrometry Article 10.1002/1097-0231(20000730)14:14<1293::AID-RCM28>3.0.CO;2-0 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.14, no.14, pp.1293 - 1300 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 14 14 1293 1300 scie scopus 000088407300021 2-s2.0-0033916358 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article gestrinone excretion study human urine HPLC GC/MS