Myung, SW Kim, S Park, JH Kim, M Lee, JC Kim, TJ 2024-01-21T15:07:42Z 2024-01-21T15:07:42Z 2021-09-04 1999-09 0003-2654 https://pubs.kist.re.kr/handle/201004/141970 A simple and rapid analytical method is presented for the determination of pethidine (meperidine) and methadone in human urine using solid-phase microextraction (SPME) and gas chromatography with nitrogen-phosphorus detection (GC-NPD). After the analytes had been partitioned between an extracting phase and the aqueous sample matrix, the needle of the coating fiber assembly was injected directly into the GC injector. The analytes were thermally desorbed in the heated injector (240 degrees C) and subsequently separated and detected by the GC-NPD system. The factors influencing the SPME method, such as the salt (NaCl) effect (15%), pH (pH 11), and equilibration time (30 min), were optimized. The calibration graphs for urine samples showed a good linearity. The detection limit was below 1 ng ml(-1) for both drugs. English ROYAL SOC CHEMISTRY Solid-phase microextraction for the determination of pethidine and methadone in human urine using gas chromatography with nitrogen-phosphorus detection Article 10.1039/a904235k 1 ANALYST, v.124, no.9, pp.1283 - 1286 ANALYST 124 9 1283 1286 N scie scopus 000083077000002 2-s2.0-0032860183 Chemistry, Analytical Chemistry Article LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY METABOLITES FLUIDS HPLC pethidine