Choi, MH Kim, JY Chung, BC 2024-01-21T16:12:50Z 2024-01-21T16:12:50Z 2021-09-03 1999-01 0003-2719 https://pubs.kist.re.kr/handle/201004/142629 A method to detect three anabolic steroids (boldenone, nandrolone and mesterolone) is presented. The anabolic steroids are isolated from equine plasma and urine by extraction with diethyl ether and C-18 Sep-Pak cartridge adsorption, respectively. The extracts obtained were derivatized with trifluoroacetyl anhydride and analyzed by GC/SIM/MS. The selected ion monitoring (SIM) mode was applied to increase the sensitivity and, when possible, the higher m/z ions were selected to improve identification. Stability of derivatives was good and compounds having hydroxy and conjugated ketone groups produced trifluoroacetyl ester derivatives that were also stable. Repeatability of the chromatographic analysis was evaluated on the basis of area repeatability, and the coefficient of variation obtained was lower than 4.4. The detection limit was 1 and 5 ng/ml for all the anabolic steroids studied in equine plasma and urine, respectively. English TAYLOR & FRANCIS INC MASS-SPECTROMETRY TESTOSTERONE TRENBOLONE Detection of anabolic steroids by GC/SIM/MS with trifluoroacetylation in equine plasma and urine Article 10.1080/00032719908542899 1 ANALYTICAL LETTERS, v.32, no.7, pp.1313 - 1322 ANALYTICAL LETTERS 32 7 1313 1322 scie scopus 000080366200004 2-s2.0-0001332175 Chemistry, Analytical Chemistry Article MASS-SPECTROMETRY TESTOSTERONE TRENBOLONE anabolic steroids GC MS trifluoroacetylation equine