Ryu, JR Lee, J Choo, S Yoon, SH Woo, ER Yu, YG 2024-01-21T16:13:31Z 2024-01-21T16:13:31Z 2021-09-03 1998-12-31 1016-8478 https://pubs.kist.re.kr/handle/201004/142641 The transmembrane protein of HIV-1, gp41, mediates fusion between membranes of the virus and target cell. Strong interaction between the helical regions in the ectodomain of gp41 has been exploited to develop a method that can detect a potential inhibitor against gp41, The N-terminus coiled-coil or the C-terminus helical sequences within the ectodomain of gp41 were inserted into the C-terminus of thioredoxin (Trx) or glutathione S-transferase (GST) to generate the fusion proteins, Trx-N and GST-C, respectively. The inserted sequences of GST-C and Trx-N cause the two proteins to interact with each other and to form a complex, Furthermore, GST-C binds specifically to the surface-coated Trx-N, and the amount of attached GST-C is detected by an ELISA assay using anti-GST antibodies, Peptides derived from the helical regions of gp41 compete with GST-C for binding to Trx-N as well as prevent the gp41-mediated cell fusion, This in vitro assay system can be applied to screening compounds that have an inhibitory activity against gp41. English SPRINGER-VERLAG SINGAPORE PTE LTD IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN FUSION MECHANISM MEMBRANE-FUSION VIRAL FUSION SOLUBLE CD4 PEPTIDE DOMAIN ACTIVATION POLYMERASE Development of an in vitro assay system for screening of gp41 inhibitory compounds Article 1 MOLECULES AND CELLS, v.8, no.6, pp.717 - 723 MOLECULES AND CELLS 8 6 717 723 scie scopus 000077860000011 2-s2.0-0032585364 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN FUSION MECHANISM MEMBRANE-FUSION VIRAL FUSION SOLUBLE CD4 PEPTIDE DOMAIN ACTIVATION POLYMERASE gp41 HIV in vitro assay inhibitor