Park, R Kim, DH Kim, MS So, HS Chung, HT Kwon, KB Ryu, DG Kim, BR 2024-01-21T16:13:38Z 2024-01-21T16:13:38Z 2021-09-03 1998-12-30 0006-291X https://pubs.kist.re.kr/handle/201004/142643 c-Src kinases and p21 Ras are known to be implicated in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated signal transduction. However, the effects of TCDD on the molecular interaction of adaptor complex in the protein tyrosine kinase signaling cascade have not been reported. The present study is designed to clarify whether TCDD modulates the molecular interactions of Shc, Cbl, Grb2, and Sos in primary rat hepatocytes. TCDD causes an electrophoretic mobility shift of Sos and increases Sos/Grba association. Tyrosine phosphorylated Shc, mainly p52, unloads to the Grb2/Sos complex upon TCDD stimulation. Interestingly, TCDD decreases the tyrosine phosphorylation level of Cbl, although Cbl recruits more Grb2 and Shc proteins by TCDD. These results indicate that TCDD modulates the molecular interaction of adaptor complex proteins including Shc, Grb2, Sos, and Chi. Furthermore, tyrosine phosphorylation of Cbl may not be critical for interaction of the protein with Grb2 and Shc in the TCDD signaling pathway in primary rat hepatocytes. (C) 1998 Academic Press. English ACADEMIC PRESS INC EPIDERMAL GROWTH-FACTOR DRUG-METABOLIZING-ENZYMES FACTOR-INDUCED ACTIVATION PROTEIN-PHOSPHORYLATION PHOSPHATIDYLINOSITOL 3-KINASE CELLS STIMULATION SIGNAL DIOXIN CRK Association of Shc, Cbl, Grb2, and Sos following treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin in primary rat hepatocytes Article 10.1006/bbrc.1998.9816 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.253, no.3, pp.577 - 581 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 253 3 577 581 scie scopus 000077846900006 2-s2.0-0032583614 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article EPIDERMAL GROWTH-FACTOR DRUG-METABOLIZING-ENZYMES FACTOR-INDUCED ACTIVATION PROTEIN-PHOSPHORYLATION PHOSPHATIDYLINOSITOL 3-KINASE CELLS STIMULATION SIGNAL DIOXIN CRK TCDD