Myung, SW Min, HK Kim, S Kim, M Cho, JB Kim, TJ 2024-01-21T16:39:32Z 2024-01-21T16:39:32Z 2022-01-11 1998-09-25 1570-0232 https://pubs.kist.re.kr/handle/201004/142851 A simple and rapid assay method for three stimulant drugs (amphetamine, methamphetamine, and dimethamphetamine) in human urine using solid-phase microextraction was developed. In solid-phase microextraction, the drugs were equilibrated between the adsorbent coated-fiber and aqueous sample matrix. After adsorption of the analytes, the fiber was directly transferred to the injector of a gas chromatograph, where the analytes were thermally desorbed and subsequently separated by the gas chromatograph and detected by mass spectrometer. The solid-phase microextraction method, which did not require solvents, was found to be a fast and simple analytical method. We optimized the solid-phase microextraction technique, for factors such as the NaCl salt effect (30%), pH effect (pH=12.4), equilibration time (30 min), desorption time (1 min) and coated-fiber type (100 mu m poly(dimethylsiloxane)) and detected the stimulants in human urine, obtained from human subjects. The detection limits of each drug were below 1-10 ng/ml. The developed method can be applied to the abused drug test. (C) 1998 Elsevier Science B.V. All rights reserved. English ELSEVIER SCIENCE BV SINGLE-COLUMN PROCEDURE BOND ELUT CERTIFY PLASMA DRUGS BLOOD Determination of amphetamine, methamphetamine and dimethamphetamine in human urine by solid-phase microextraction (SPME) gas chromatography mass spectrometry Article 10.1016/S0378-4347(98)00304-1 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.716, no.1-2, pp.359 - 365 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 716 1-2 359 365 scie scopus 000076482100041 2-s2.0-0032566705 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article SINGLE-COLUMN PROCEDURE BOND ELUT CERTIFY PLASMA DRUGS BLOOD amphetamine methamphetamine dimethamphetamine