Choi, MJ Song, EY Chung, TW 2024-01-21T17:05:14Z 2024-01-21T17:05:14Z 2021-09-03 1998-06 0253-6269 https://pubs.kist.re.kr/handle/201004/143050 A sensitive enzyme immunoassay for serum TSH has been developed utilizing the tight binding between biotin and avidin, and three layered protein polystyrene beads as solid phase. To increase binding capacity of TSH and sensitivity of the assay, the polystyrene beads were coated sequentially with mouse immunoglobulin as first layer, rabbit antimouse immunoglobulin as second layer and monoclonal anti-TSH as third layer. A serum sample was incubated simultaneously with a monoclonal anti-TSH immobilized polystyrene beads and a second monoclonal anti-TSH covalently attached to biotin. After washing, the antibody bound serum TSH-anti-TSH-biotin complex is reacted with horseradish peroxidase (HRP)-labeled avidin. Following a second wash, the bound HRP activity was measured colorimetrically. Reproducible results were obtained within 4 hours for serum TSH in the range between 0 mu lU/ml and 50 mu lU/ml with detection limit of 0.1 mu lU per test. English PHARMACEUTICAL SOCIETY KOREA A biotin-avidin labeled enzyme immunoassay for the quantitation of serum TSH using protein-layered solid phase Article 10.1007/BF02975280 1 ARCHIVES OF PHARMACAL RESEARCH, v.21, no.3, pp.231 - 235 ARCHIVES OF PHARMACAL RESEARCH 21 3 231 235 scie scopus 000074151900002 2-s2.0-0032083235 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article biotin-avidin EIA TSH trilayered solid phase