Cho, NH Seong, SY Chun, KH Kim, YH Kwon, IC Ahn, BY Jeong, SY 2024-01-21T17:09:08Z 2024-01-21T17:09:08Z 2021-09-03 1998-04-30 0168-3659 https://pubs.kist.re.kr/handle/201004/143116 A novel mucosal immunization was examined using biocompatible and biodegradable alginate microspheres containing a conjugate of polysaccharide antigen and cholera toxin B subunit (CTB). In order to prepare the alginate microspheres with diameters of less than 5 mu m, a new diffusion-controlled interfacial gelation technique was developed. Also, in order to improve the mucosal immune response, a pneumococcal capsular polysaccharide type 19 (PS19) was conjugated to the CTB (PS19-CTB). This conjugate was subsequently encapsulated into the alginate microspheres. The loading content of PS19-CTB to the alginate microspheres was 60%. An in vitro sustained release pattern was observed with the antigen-loaded microspheres, showing 80% antigen release within one day. Mucosal and systemic immunities following oral immunization with the alginate microspheres were studied. Balb/c mice were immunized perorally three times at intervals of two weeks. Peroral immunization with 25 mu g of PS19-CTB entrapped in the alginate microspheres evoked both the mucosal IgA and systemic IgM responses to PS19 in small intestine and in sera, respectively. The results suggest that both the mucosal and systemic antibody responses could be induced by oral administration of the PS19-CTB antigen entrapped in alginate microspheres. (C) 1998 Elsevier Science B.V. English ELSEVIER SCIENCE BV PARTICLE-SIZE POLY(LACTIDE-CO-GLYCOLIDE) MICROPARTICLES DISPERSION POLYMERIZATION CONTROLLED-RELEASE SUPPRESSOR CELLS PEYERS PATCHES INDUCTION RESPONSES ANTIGEN VACCINATION Novel mucosal immunization with polysaccharide-protein conjugates entrapped in alginate microspheres Article 10.1016/S0168-3659(97)00255-1 1 JOURNAL OF CONTROLLED RELEASE, v.53, no.1-3, pp.215 - 224 JOURNAL OF CONTROLLED RELEASE 53 1-3 215 224 scie scopus 000073522600023 2-s2.0-0032580501 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article; Proceedings Paper PARTICLE-SIZE POLY(LACTIDE-CO-GLYCOLIDE) MICROPARTICLES DISPERSION POLYMERIZATION CONTROLLED-RELEASE SUPPRESSOR CELLS PEYERS PATCHES INDUCTION RESPONSES ANTIGEN VACCINATION alginate microsphere mucosal immunization pneumococcal pneumonia oral vaccine