Choi, J Kim, C Choi, MJ 2024-01-21T17:16:05Z 2024-01-21T17:16:05Z 2021-09-05 1998-02-13 0378-4347 https://pubs.kist.re.kr/handle/201004/143236 An accurate, simple and rapid immunoassay is demonstrated for the detection of methamphetamine in urine by capillary electrophoresis (CE) with laser-induced fluorescence (LIF). An aminobutyl derivative of methamphetamine was conjugated with proteins, and used as an immunogen to produce antibodies for the essay. The methamphetamine derivative was also labeled with fluorescein isothiocyanate (FITC) to compete with free methamphetamine in the sample for the antibody binding site. Levels of free and antibody-bound FITC-labeled methamphetamine were monitored by performing CE-LIF using an untreated fused-silica column. This competitive immunoassay used antiserum instead of purified antibody or antibody fragment, yet was found to have good precision with a sensitivity of lower than 20 ng/ml. Various antibodies were also screened, and cross-reactivity of anti-MA antibody with methamphetamine analogues were also investigated. The results indicate that CE-LIF-based immunoassay is a powerful tool for the screening and characterization of antibody and may have possible applications in the detection of abused drugs in urine. (C) 1998 Elsevier Science B.V. English ELSEVIER SCIENCE BV POLARIZATION FLUOROIMMUNOASSAY GAS-CHROMATOGRAPHY HUMAN URINE IMMUNOASSAY AMPHETAMINE DRUG CONFIRMATION Immunological analysis of methamphetamine antibody and its use for the detection of methamphetamine by capillary electrophoresis with laser-induced fluorescence Article 10.1016/S0378-4347(97)00527-6 1 JOURNAL OF CHROMATOGRAPHY B, v.705, no.2, pp.277 - 282 JOURNAL OF CHROMATOGRAPHY B 705 2 277 282 scie scopus 000072178500012 2-s2.0-0032512662 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article POLARIZATION FLUOROIMMUNOASSAY GAS-CHROMATOGRAPHY HUMAN URINE IMMUNOASSAY AMPHETAMINE DRUG CONFIRMATION urine methamphetamine