Choi, MH Chung, BC Kim, M Choi, J Kim, Y 2024-01-21T17:34:02Z 2024-01-21T17:34:02Z 2022-01-11 1998-01 0951-4198 https://pubs.kist.re.kr/handle/201004/143298 A gas chromatography/mass spectrometry method is described which uses negative ion chemical ionization and tandem mass spectrometry for the determination of anabotic steroid metabolites. Four anabolic steroid metabolites to be derivatized by Pentafluoropropionic anhydride (PFPA) were determined using gas chromatography/mass spectrometry (GC/MS) with negative chemical ionization (NCI) and NCI/MS/MS. The repeatability and reproducibility of this procedure were in the range of 5.3-9.7% and 6.1-10.2%, respectively. This method of derivatization with PFPA for NCI and NCI/MS/MS was useful to determine four metabolites of nandrolone, dromostanolone, methenolone and boldenone, The derivatized metabolites of boldenone could be detected to 2 ppb and the other three steroids could be detected to 25 ppb in urine at a signal to-noise ratio of S/N = 3, (C) 1998 John Wiley & Sons, Ltd. English JOHN WILEY & SONS LTD URINARY METABOLITES IDENTIFICATION INTERFERENCES CONFIRMATION MESTEROLONE METHENOLONE SUBSTANCES DRUGS Determination of four anabolic steroid metabolites by gas chromatography mass spectrometry with negative ion chemical ionization and tandem mass spectrometry Article 10.1002/(SICI)1097-0231(19981130)12:22<1749::AID-RCM395>3.0.CO;2-S 1 RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.12, no.22, pp.1749 - 1755 RAPID COMMUNICATIONS IN MASS SPECTROMETRY 12 22 1749 1755 scie scopus 000077057000016 2-s2.0-0001245340 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article URINARY METABOLITES IDENTIFICATION INTERFERENCES CONFIRMATION MESTEROLONE METHENOLONE SUBSTANCES DRUGS