Suh, JW Lee, SH Chung, BC 2024-01-21T17:45:42Z 2024-01-21T17:45:42Z 2021-09-01 1997-12 0009-9147 https://pubs.kist.re.kr/handle/201004/143500 We combined column and partition chromatography to isolate, purify, and quantify biological organic acids in urine and cerebrospinal fluid (CSF). Urine and CSF samples were introduced onto a preconditioned cation-exchange column (Dowex 50W x 8 resin) to remove the biological interferences. The effluent with water was extracted with ethyl acetate two times (pH 1 and 3) and the organic acids were quantitatively converted into their trimethylsilyl derivatives for detection by gas chromatography-mass spectrometry. The good qualify-control data were obtained through precision and accuracy tests. Inter- and intraassay CVs were 0.01-10.2% and 0.02-12.2%, respectively. Analytical recoveries compared favorably with results from the commonly used solvent extraction method. This method was used for the measurement of the 14 organic acids in the urine and CSF of healthy volunteers. The values obtained were in the range of the published data. English AMER ASSOC CLINICAL CHEMISTRY GC-MS determination of organic acids with solvent extraction after cation-exchange chromatography Article 1 CLINICAL CHEMISTRY, v.43, no.12, pp.2256 - 2261 CLINICAL CHEMISTRY 43 12 2256 2261 scie scopus 000071133800006 2-s2.0-0001027401 Medical Laboratory Technology Medical Laboratory Technology Article GC/MS organic acids cation exchange chromatography