Choi, MJ Choi, J Yoon, DY Park, J Eremin, SA 2024-01-21T18:34:49Z 2024-01-21T18:34:49Z 2021-09-05 1997-04 0918-6158 https://pubs.kist.re.kr/handle/201004/143873 A homogeneous fluorescence polarization immunoassay (FPIA) was developed to measure levels of progesterone in urine using a TDx analyzer in photocheck mode (Abbott Labs). Two tracers of ethylenediamine fluorescein thiocarbamyl (EDF) were employed; one was synthesized from 11 alpha-hydroxyhemisuccinate progesterone (Prog-11OH-HS) and the other was synthesized from 3-(o-carboxymethyl)oxime progesterone (Prog-3CMO), Each derivative of progesterone was conjugated with bovine serum albumin and used as an immunogen which produced monoclonal antibody clone 15A (MAb 15A, anti-Prog-11OH-HS) and clone 2B7 (MAb 2B7, anti-Prog-3CMO), respectively, Different combinations of tracers and antibodies were investigated in the FPIA system, Similar sensitivity was observed when using the pair, MAb 2B7 and its homologous tracer, Prog-3CMO-EDF, or MAb 15A and its homologous tracer, Prog-11OH-HS-EDF, In this immunoassay, no separation step was required and the total time for an assay of 10 samples was approximately 7 min, The progesterone detection limit in a 10 mu l sample was 3 ng/ml, The cross-reactivity results indicate that the A-, B- and D-ring of a steroid are buried in the binding pocket of MAb 15A, while the C-ring faced outward, resulting in cross-reactivity with 11-alpha hydroxy progesterone, The A-, B- and C-ring of a steroid of MAb 2B7, in contrast, are buried deep in the pocket leaving the D-ring facing outward, resulting in some different degrees of cross-reactivity with C17 position substituted steroids. English PHARMACEUTICAL SOC JAPAN MONOCLONAL-ANTIBODIES SOLID-PHASE FLUOROIMMUNOASSAY SPECIFICITY METHAMPHETAMINE SERUM Fluorescence polarization immunoassay of progesterone Article 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.20, no.4, pp.309 - 314 BIOLOGICAL & PHARMACEUTICAL BULLETIN 20 4 309 314 scie scopus A1997WU87900003 2-s2.0-0342710354 Pharmacology & Pharmacy Pharmacology & Pharmacy Article MONOCLONAL-ANTIBODIES SOLID-PHASE FLUOROIMMUNOASSAY SPECIFICITY METHAMPHETAMINE SERUM fluorescence polarization immunoassay progesterone fluorescence immunoassay immunogen tracer