Woo, ER Yoon, SH Kwak, JH Kim, HJ Park, H 2024-01-21T18:38:00Z 2024-01-21T18:38:00Z 2021-09-05 1997-03 0944-7113 https://pubs.kist.re.kr/handle/201004/143928 Various assay methods have been developed to evaluate the effectiveness of substances against human immunodeficiency virus (HIV). One of them is the Syncytia formation inhibition assay, which is based on the inhibition of the interaction between the HIV-1 envelope protein gp 120 and the cellular membrane protein CD4. A variation of this assay using recombinant virus vPE 16 and CD4(+) HeLa cell was developed to find anti-HIV compounds in natural products that inhibit gp 120-CD4 binding. VPE 16 expresses glycoprotein gp 160, which is glycosylated then processed into gp 120 and gp 41 on its envelope. A total of 50 plant extracts were screened with this system. Extracts from Calicarpa japonica and Sedum sarmentosum were among those that showed strong inhibition of the gp 120-CD4 interaction. English GUSTAV FISCHER VERLAG Inhibition of gp 120-CD4 interaction by various plant extracts Article 10.1016/S0944-7113(97)80028-1 1 PHYTOMEDICINE, v.4, no.1, pp.53 - 58 PHYTOMEDICINE 4 1 53 58 N scie scopus A1997WX04000008 2-s2.0-0002386888 Plant Sciences Chemistry, Medicinal Integrative & Complementary Medicine Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Integrative & Complementary Medicine Article IMMUNODEFICIENCY-VIRUS TYPE-1 PLAQUE-ASSAY ANTI-HIV-1 ACTIVITY ANTIVIRAL COMPOUNDS GP120-CD4 BINDING HTLV-III/LAV CELLS HIV-1 AIDS RETROVIRUSES human immunodeficiency virus (HIV) gp120 CD4 interaction antiviral agents natural products vPE 16 CD4(+) HeLa