Park, KD Suzuki, K Lee, WK Lee, JE Kim, YH Sakurai, Y Okano, T 2024-01-21T19:15:05Z 2024-01-21T19:15:05Z 2022-01-10 1996-09 1058-2916 https://pubs.kist.re.kr/handle/201004/144328 Polyurethane (PU) surfaces were modified by coupling polyethylene glycol (PEG; molecular weight, 1,000) chains carrying different terminal groups (PU-PEC 1K-OH, PU-PEG1K-NH2, PU-PEG1K-SO3) and longer PEG chains (MW, 3,350; PU-PEG3.4K-OH). The modified PU surfaces have the same PEG (1K) chain density. Surface induced platelet activation was evaluated by measuring cytoplasmic free calcium concentration in platelets contacting modified surfaces, and platelet adhesion onto modified surfaces was investigated in vitro. Cytoplasmic free calcium levels in platelets contacting PU-PEG-SO3 remained relatively constant, in contrast to the significant increase observed for PU-PEC-NH2, PU-PEG-OH, and control PU surfaces. The degree of platelet adhesion clearly demonstrates that all PEG graft surfaces prevented platelet adhesion. Among PEG1K surfaces, PU-PEG-SO3 shows the lowest platelet adhesion. In the case of relatively longer PEG grafted surfaces (PU-PEG3.4K-OH and PU-PEG3.4K-Hep), both surfaces were found to prevent the increase in both cytoplasmic free calcium and platelet adhesion. These results suggest that longer PEG chain grafting is more effective than shorter grafting in preventing platelet activation and adhesion because of the highly dynamic movement of hydrated PEG chains at the interface. In addition, in vitro platelet interaction is dependent upon terminal groups of PEC chains on PEG1K series surfaces. English LIPPINCOTT-RAVEN PUBL Platelet adhesion and activation on polyethylene glycol modified polyurethane surfaces - Measurement of cytoplasmic calcium Article 10.1097/00002480-199609000-00117 1 ASAIO JOURNAL, v.42, no.5, pp.M876 - M881 ASAIO JOURNAL 42 5 M876 M881 scie scopus A1996VP50800116 2-s2.0-0346290930 Engineering, Biomedical Transplantation Engineering Transplantation Article