Rhim, H Toth, PT Miller, RJ 2024-01-21T19:33:43Z 2024-01-21T19:33:43Z 2021-09-01 1996-07 0007-1188 https://pubs.kist.re.kr/handle/201004/144411 1 High-threshold Ca2+ channel currents were measured every 15 s following a 200 ms voltage step from -80 mV to 0 mV in order to study the coupling mechanism between neurotransmitter receptors and Ca2+ channels in neurones acutely isolated from the nucleus tractus solitarius (NTS) of the rat. 2 Application of 30 pM baclofen (GABAB receptor agonist) caused 38.9+/-1.2% inhibition of the peak inward Ba2+ current (I-Ba2+) in most NTS cells tested (n = 85 of 88). Somatostatin, 300 nM, also reduced I-Ba2+ by 31.3+/-1.6% in 53 cells of 82 tested. 3 Activation of mu-opioid-, GABA(B)- or somatostatin-receptors inhibited both N- and P/Q-type Ca2+ channels. 4 The inhibition of Ca2+ currents by DAMGO (mu-opioid receptor agonist), baclofen and somatostatin was seduced by treatment with pertussis toxin and partially relieved by application of a 50 ms conditioning prepulse to +80 mV. This suggests that a pertussis toxin-sensitive G-protein was involved in the neurotransmitter-mediated action in the observed inhibition of Ca2+ currents. 5 Intracellular loading with an antiserum raised against the amino terminus of G(o alpha) (CC/2) markedly attenuated the somatostatin-induced inhibition, but did not block the DAMGO- and baclofen-induced inhibition. 6 These findings suggest at least two different pertussis toxin-sensitive G-protein-mediated pathways are involved in receptor-induced inhibition of Ca2+ currents in the NTS. English STOCKTON PRESS GAMMA-AMINOBUTYRIC-ACID GTP-BINDING-PROTEIN CHICK SENSORY NEURONS ROOT GANGLION NEURONS MU-OPIOID RECEPTOR SYMPATHETIC NEURONS SYNAPTIC TRANSMISSION CA2+ CURRENTS VOLTAGE DEPENDENCE BETA-SUBUNIT Mechanism of inhibition of calcium channels in rat nucleus tractus solitarius by neurotransmitters Article 10.1111/j.1476-5381.1996.tb15543.x 1 BRITISH JOURNAL OF PHARMACOLOGY, v.118, no.6, pp.1341 - 1350 BRITISH JOURNAL OF PHARMACOLOGY 118 6 1341 1350 scie scopus A1996UY15700003 Pharmacology & Pharmacy Pharmacology & Pharmacy Article GAMMA-AMINOBUTYRIC-ACID GTP-BINDING-PROTEIN CHICK SENSORY NEURONS ROOT GANGLION NEURONS MU-OPIOID RECEPTOR SYMPATHETIC NEURONS SYNAPTIC TRANSMISSION CA2+ CURRENTS VOLTAGE DEPENDENCE BETA-SUBUNIT mu-opioid receptor GABA(B) receptor somatostatin pertussis toxin G-protein brainstem autonomic nervous system