Kim, M.-S. KIM, BYUNG HONG Chung, Eun Young 이철훈 2024-01-21T20:14:08Z 2024-01-21T20:14:08Z 2021-09-02 1996-01 0257-2389 https://pubs.kist.re.kr/handle/201004/144866 A facultative anaerobe Enterococcus faecium 19-46-4 was used to study the production of an antimicrobial substance in anaerobic conditions. Major part of the antibiotic activity was found in the culture filtrate of the bacterium. The active compound was extracted by an equal volume of iso-butanol and concentrated in vacuo (at 50°C) before purification by C-18 liguid column chromatography and HPLC. A chromatographically pure compound was obtained by two passages of HPLC columns, The compound appeared as a pale-yellow powder. The yield was about 2.5 mg 1-1 culture filtrate. The compound was named as KIST 194. KIST 194 were identified as cholic acid (3α, 7α, 12α-trihydroxy-5β-cholan 24-oic acid) based on its physicochemical properties determined by UV, IR, 1H-NMR, 13C-NMR, EI-MS and LC-MS. Korean Cholic acid produced by enterococcus faecium 19-46-4 Article 1 Korean Journal of Applied Microbiology and Biotechnology, v.24, no.5, pp.540 - 545 Korean Journal of Applied Microbiology and Biotechnology 24 5 540 545 N scopus 2-s2.0-29244440417 Article Antibacterial substances Cholic acid Enterococcits faecium Lactic acid bacteria