LEE, YW ROW, KH SO, MS POLUNINA, IA LARIN, AV 2024-01-21T21:14:33Z 2024-01-21T21:14:33Z 2021-09-02 1995-01 0148-3919 https://pubs.kist.re.kr/handle/201004/145424 Comparison of approaches describing retention of deoxynucleosides (dCyd, dUrd, dGuo, dThd, and dAdo) as a function of the mobile phase composition has been performed. The volume fraction (F) of organic modifier was changed from 0.05 to 0.30, and to 0.12 for methanol and acetonitrile, respectively. Linear regression has been carried out for the following equations: log k' = log k'(w) - S X F (1), log k' = H + Klog(1/F) (2), k' = A + B(1/F) (3), where k' is capacity factor, and log k'(w), S, H, K, A and B are experimental coefficients. The best fits mere obtained for the eqns. (1) and (3), and the poorest correlations were obtained for eqn. (2). The Slope ratios B-methanol/B-acetonitrile and S-acetonitrile/S-methanol are changed in comparatively narrow ranges with average values of 2.86 and 2.30, respectively. Unlike the slope S of eqn. (1), the slope B of eqn. (3) can characterize the properties of solute and organic modifier simultaneously. For each solute, the intercepts A calculated for acetonitrile and methanol as organic modifiers are coincident closely. It is shown the validity of the coefficients of eqn. (3) to express the deoxyribonucleoside properties. English MARCEL DEKKER INC PERFORMANCE LIQUID-CHROMATOGRAPHY REVERSED-PHASE HPLC RETENTION OF DEOXYRIBONUCLEOSIDES AS A FUNCTION OF MOBILE-PHASE COMPOSITION Article 10.1080/10826079508010434 1 JOURNAL OF LIQUID CHROMATOGRAPHY, v.18, no.15, pp.3077 - 3089 JOURNAL OF LIQUID CHROMATOGRAPHY 18 15 3077 3089 scie scopus A1995RW72600009 2-s2.0-0029153720 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article PERFORMANCE LIQUID-CHROMATOGRAPHY reversed-phase HPLC mobile phase composition orgarnic modifier