YOON, DY SONG, EY KWON, DH CHOI, MJ BYUN, SM CHOE, IS CHUNG, TW 2024-01-21T21:14:36Z 2024-01-21T21:14:36Z 2021-09-02 1995-01 0197-1522 https://pubs.kist.re.kr/handle/201004/145425 A simple method of visual membrane enzyme-immunoassay (EIA) for the detection of progesterone is described. When two types of progesterone-horseradish peroxidase (HRP) tracers were challenged for binding, in the presence of progesterone, to the monoclonal antiprogesterone antibody, 15A, coated on the microtitre plate, the HRP conjugated at the C-3 position (A-ring) of progesterone competed more effectively with progesterone to the binding site of the monoclonal antibody (mAb) than HRP conjugated at the C-11 position of the C-ring. By using this combination of mAb, 15A, and progesterone-3(O-carboxymethyloxime)-HRP (P-3CMO-HRP), we developed a visual membrane EIA system in which free progesterone in the sample could be quantified by the degree of color development. In this system, free progesterone competed with P-3CMO-HRP for binding sites of mAb immobilized on the nitrocellulose membrane. The stable grey color was formed on the surface of membrane for progesterone-negative and no color for progesterone-positive sample using 3,3'-diaminobenzidine (DAB) with Co2+ as an insoluble substrate solution. To examine whether tetramethylbenzidine (TMB) can substitute for DAB in membrane EIA, an experiment was conducted where TMB was used as an insoluble substrate. English MARCEL DEKKER INC USE OF PROGESTERONE-3(O-CARBOXYMETHYL OXIME)-HORSERADISH PEROXIDASE IN A SENSITIVE MICROTITRE-PLATE EIA AND ITS APPLICATION TO A VISUAL MEMBRANE EIA OF PROGESTERONE Article 10.1080/15321819508013554 1 JOURNAL OF IMMUNOASSAY, v.16, no.2, pp.137 - 153 JOURNAL OF IMMUNOASSAY 16 2 137 153 N scie scopus A1995QT31600002 2-s2.0-0028931371 Biochemistry & Molecular Biology Immunology Medical Laboratory Technology Biochemistry & Molecular Biology Immunology Medical Laboratory Technology Article ENZYME-IMMUNOASSAY URINE METHAMPHETAMINE PREGNANCY BENZIDINE PROGESTERONE VISUAL MEMBRANE IMMUNOASSAY