CHOI, MJ GOROVITS, BM CHOI, J SONG, EY NAM, KS PARK, J 2024-01-21T21:35:39Z 2024-01-21T21:35:39Z 2021-09-02 1994-07 0918-6158 https://pubs.kist.re.kr/handle/201004/145550 A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. Tracers of horseradish peroxidase-labeled methamphetamine (MA-HRP) and amphetamine (A-HRP) derivatives were prepared for this purpose. Significant differences in antibody specificity were found between the two tracers. Based on the results of this study, a pair of an antibody and a tracer was selected and a membrane enzyme immunoassay (EIA) was developed utilizing the competitive binding between methamphetamine and the drug-HRP tracer. UltraBind membrane (0.45 mum) was used as the solid matrix to which the antibody was attached. Using diaminobenzidine substrate with Co2+ ion, a stable grey color appeared on the surface of membrane for MA-negative urine samples. No color appeared for MA-positive urine with a cut-off level of 0.8 ppm. English PHARMACEUTICAL SOC JAPAN REAGENT POLARIZATION FLUOROIMMUNOASSAY URINE A VISUAL MEMBRANE IMMUNOASSAY FOR THE DETECTION OF METHAMPHETAMINE USING AN ENZYME-LABELED TRACER DERIVED FROM METHAMPHETAMINE AND AMPHETAMINE Article 1 BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.17, no.7, pp.875 - 880 BIOLOGICAL & PHARMACEUTICAL BULLETIN 17 7 875 880 scie scopus A1994NY40000001 2-s2.0-0028122655 Pharmacology & Pharmacy Pharmacology & Pharmacy Article REAGENT POLARIZATION FLUOROIMMUNOASSAY URINE METHAMPHETAMINE AMPHETAMINE IMMUNOGEN TRACER INFLUENCE VISUAL MEMBRANE IMMUNOASSAY