Park, Ki Dong Yun, Ju Young Han, Dong Keun Jeong, Seo Young Kim, Young Ha Choi, Kyu Suk Kim, Hyoung Mook Kim, Hack Joo Kim, Kwang Taek 2024-01-21T22:10:45Z 2024-01-21T22:10:45Z 2021-09-02 1994-01 1058-2916 https://pubs.kist.re.kr/handle/201004/145908 Biologic porcine tissue was modified by coupling sulfonated polyethyl- eneoxide (PEO-SO3) and the effect of modification on calcification was evaluated in vitro and in vivo. The modification process involves grafting PEO-SO3 to porcine valve leaflet either by carbodiimide (EDC) activation or by direct coupling using glutaraldehyde. Thermal property, measured by differential scanning calorimetry, showed that the shrinkage temperature of modified tissue increased compared with control tissue and fresh tissue, suggesting increased thermal stability. Resistance to collagenase digestion revealed that modified tissues have greater resistance to enzyme digestion than do control tissues. In vitro calcification showed that modified tissues have less calcium deposition than do control tissues. In vivo calcification, using a rat subcutaneous implantation model, also showed less calcification of modified tissue than that of control. The resistance of modified tissue to collagenase, higher shrinkage temperature, and reduced calcification, when compared with control tissue, attest to the usefulness of this chemical modification for implantable biologic tissue.Biologic porcine tissue was modified by coupling sulfonated polyethyl-eneoxide (PEO-SO3) and the effect of modification on calcification was evaluated in vitro and in vivo. The modification process involves grafting PEO-SO3 to porcine valve leaflet either by carbodiimide(EDC) activation or by direct coupling using glutaraldehyde. Thermal property, measured by differential scanning calorimetry, showed that the shrinkage temperature of modified tissue increased compared with control tissue and fresh tissue, suggesting increased thermal stability. Resistance to collagenase digestion revealed that modified tissues have greater resistance to enzyme digestion than do control tissues. In vitro calcification showed that modified tissues have less calcium deposition than do control tissues. In vivo calcification, using a rat subcutaneous implantation model, also showed less calcification of modified tissue than that of control. The resistance of modified tissue to collagenase, higher shrinkage temperature, and reduced calcification, when compared with control tissue, attest to the usefulness of this chemical modification for implantable biologic tissue. English J.B. Lippincott Co, Hagerstown Calcification (biochemistry) Calorimetry Chemical modification Composition effects Grafting (chemical) Physiological models Thermal effects Thermodynamic stability Tissue Biologic porcine tissue Carbodiimide activation Collagenase digestion Coupling sulfonated polyethyl-eneoxide Differential scanning calorimetry Enzyme digestion Glutaraldehyde Implantable biologic tissue Shrinkage temperature Implants (surgical) collagenase cyanamide macrogol derivative animal experiment animal tissue article calcification chemical modification differential scanning calorimetry enzyme activity implantation nonhuman postoperative complication rat swine thermostability Chemical modification of implantable biologic tissue for anti- calcification Article 10.1097/00002480-199407000-00026 1 ASAIO Journal, v.40, no.3, pp.M377 - M382 ASAIO Journal 40 3 M377 M382 scopus 2-s2.0-0028467536 Article Calcification (biochemistry) Calorimetry Chemical modification Composition effects Grafting (chemical) Physiological models Thermal effects Thermodynamic stability Tissue Biologic porcine tissue Carbodiimide activation Collagenase digestion Coupling sulfonated polyethyl-eneoxide Differential scanning calorimetry Enzyme digestion Glutaraldehyde Implantable biologic tissue Shrinkage temperature Implants (surgical) collagenase cyanamide macrogol derivative animal experiment animal tissue article calcification chemical modification differential scanning calorimetry enzyme activity implantation nonhuman postoperative complication rat swine thermostability anti-calcification tissue sulfonated PEO grafting