Sim, Sang Jun Chang, Ho Nam Jang, Ryol Liu Jung, Kyung Hee 2024-01-21T22:10:53Z 2024-01-21T22:10:53Z 2021-09-02 1994-01 0922-338X https://pubs.kist.re.kr/handle/201004/145910 Various hairy root clones were derived by transforming two kinds of Cathanranthus roseus plants (Catharanthus roseus cvs. Little Linda and Little Delicata) with Agrobacterium rhizogenes ATCC 15834. Hairy root growth and indole alkaloid production were then investigated to select a high-yielding hairy root clone. Among three Amberlite resins (XAS-2, XAD-4, and ajmalicine from hairy root cultures, with an increase in total production. To enhance alkaloid production and secretion, a permeabilizing agent (dimethyl sulfoxide) and a fungal elicitor to provide physical and biochemical stress, respectively, together with in situ adsorption. Dimethyl sulfoxide (0.5% v/v) treatment with in situ adsorption using XAD-7 was found appropriate for releasing indole alkaloids from hairy roots without affecting cell viability. In addition, fungal elicitation by Penicillium sp. enhanced both alkaloid production and secretion. By combining in situ adsorption sequentially with these techniques, the release ratio of catharanthine and ajmalicine was enhanced up to 20 and 70%, respectively, which was 3.4 and 2 times higher than that obtained with in situ adsorption by XAD-7 alone. Over a 27-d culture period, the total amounts of catharanthine and ajmalicine produced were 67 and 30.15 mg/l, respectively. These results indicated that the in situ adsorption sequentially applied with permeabilization and fungal elicitation have a synergistic effect on the production and secretion of indole alkaloids. ？ 1994. English Adsorption Biosynthesis Cell immobilization Extraction Fungi Growth kinetics Mechanical permeability Metabolites Agrobacterium rhizogenes Ajmalicine Alkaloids Catharanthine Catharanthus roseus Hairy root cultures Plant cell culture ajmalicine amberlite xad resin catharanthine dimethyl sulfoxide indole alkaloid unclassified drug adsorption article biosynthesis fungus nonhuman permeabilization plant root secretion Agrobacterium rhizogenes Catharanthus roseus Fungi Penicillium sp. Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus: Effects of in situ adsorption, fungal elicitation and permeabilization Article 10.1016/0922-338X(94)90295-X 1 Journal of Fermentation and Bioengineering, v.78, no.3, pp.229 - 234 Journal of Fermentation and Bioengineering 78 3 229 234 scopus 2-s2.0-0027984628 Article Adsorption Biosynthesis Cell immobilization Extraction Fungi Growth kinetics Mechanical permeability Metabolites Agrobacterium rhizogenes Ajmalicine Alkaloids Catharanthine Catharanthus roseus Hairy root cultures Plant cell culture ajmalicine amberlite xad resin catharanthine dimethyl sulfoxide indole alkaloid unclassified drug adsorption article biosynthesis fungus nonhuman permeabilization plant root secretion Agrobacterium rhizogenes Catharanthus roseus Fungi Penicillium sp. indole alkaloids hairy root Catharanthus roseus in situ adsorption permeabilization fungal elicitation