YOO, YS KIM, YS JHON, GJ PARK, JS 2024-01-21T22:14:28Z 2024-01-21T22:14:28Z 2021-09-02 1993-10-22 0021-9673 https://pubs.kist.re.kr/handle/201004/145968 Gangliosides are glycosphingolipids containing sialic acid. These glycolipids have been suggested to play important roles in biological processes such as cell growth, differentiation and malignant transformation. Based on these proposed biological functions, gangliosides can be used as diagnostic tools and therapeutics for various human diseases. In this study, capillary zone electrophoresis (CZE) was used to determine the major gangliosides G(M1), G(D1a), G(D1b) and G(T1b) in mammalian brains, in addition to G,, and Lac. Enhancement of selectivity and efficiency of separation was obtained by using 50 mM borate-phosphate buffer containing 16.5 mM alpha-cyclodextrin (alpha-CD). Under this condition, several forms of gangliosides were successfully separated from extracts of deer antler, apricot seed and rat brain. The results demonstrate that the CD-modified CZE is a useful method for detecting glycolipids from various biological matrices. English ELSEVIER SCIENCE BV PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROKINETIC CHROMATOGRAPHY MICELLAR ISOTACHOPHORESIS RESOLUTION PROTEINS BEHAVIOR SEPARATION OF GANGLIOSIDES USING CYCLODEXTRIN IN CAPILLARY ZONE ELECTROPHORESIS Article 1 JOURNAL OF CHROMATOGRAPHY A, v.652, no.2, pp.431 - 439 JOURNAL OF CHROMATOGRAPHY A 652 2 431 439 scie scopus A1993MW22000017 2-s2.0-0027366654 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article PERFORMANCE LIQUID-CHROMATOGRAPHY ELECTROKINETIC CHROMATOGRAPHY MICELLAR ISOTACHOPHORESIS RESOLUTION PROTEINS BEHAVIOR