Park, S.-J. Kim, Y.-J. Pyo, H.-S. Park, J. 2024-01-22T00:40:04Z 2024-01-22T00:40:04Z 2022-01-10 1990-01 0146-4760 https://pubs.kist.re.kr/handle/201004/147271 The method for simultaneous determination and confirmation of nine corticosteroids in urine by high-performance liquid chromatography with a diode array detector and thermospray liquid chromatography-mass spectrometry was studied. HPLC was performed on a C18 column with a gradient mobile phase system of water and acetonitrile. The calibration curve was linear from 20 ng/mL to 1.0 μg/mL for each corticosteroid, and the detection limit was 10 ng/mL in 5 mL of urine. The extraction recovery of each corticosteroid from the spiked urine was equal to or greater than 85% by liquid-liquid extraction (LLE) at pH 9 using diethyl ether and approximately 79% or more by solid-phass extraction (SPE) with a Sep-Pak C18 cartridge. The mass spectra obtained with the positive ion mode showed protonated molecular species [M + H] +, ammonium adduct ion [M + NH4] +, [MH60]+, [MH30]+, and [MH18]+, The LC/MS detection limits ranged from 10 to 50 ng in the scan mode and from 1 to 5 ng in the selected ion monitoring (SIM) mode. ？ 1990 Oxford University Press. English 11alpha hydroxyprogesterone corticosteroid corticosterone cortisone deoxycorticosterone doping control hydrocortisone prednisolone prednisone triamcinolone triamcinolone acetonide unclassified drug article doping drug analysis high performance liquid chromatography human liquid chromatography mass spectrometry methodology priority journal urine Adrenal Cortex Hormones Chromatography, High Pressure Liquid Chromatography, Liquid Doping in Sports Glucocorticoids, Synthetic Human Hydroxyprogesterones Spectrum Analysis, Mass Substance Abuse Detection Analysis of corticosteroids in urine by hplc and thermospray lc/ms Article 10.1093/jat/14.2.102 1 Journal of Analytical Toxicology, v.14, no.2, pp.102 - 108 Journal of Analytical Toxicology 14 2 102 108 scopus 2-s2.0-0025262204 Article 11alpha hydroxyprogesterone corticosteroid corticosterone cortisone deoxycorticosterone doping control hydrocortisone prednisolone prednisone triamcinolone triamcinolone acetonide unclassified drug article doping drug analysis high performance liquid chromatography human liquid chromatography mass spectrometry methodology priority journal urine Adrenal Cortex Hormones Chromatography, High Pressure Liquid Chromatography, Liquid Doping in Sports Glucocorticoids, Synthetic Human Hydroxyprogesterones Spectrum Analysis, Mass Substance Abuse Detection corticosteroids urine thrmospray LC/MS HPLC