Chung, B. Choo, H.-Y.P. Kim, T. Eom, K. Kwon, O. Suh, J. Yang, J. Park, J. 2024-01-22T00:40:33Z 2024-01-22T00:40:33Z 2021-09-02 1990-01 0146-4760 https://pubs.kist.re.kr/handle/201004/147279 This study describes the use of gas chromatography/mass spectrometry with selected ion monitoring to screen 18 anabolic steroids banned by the International Olympic Committee. These anabolic steroids are analyzed in two fractions depending on their excretion pattern: nonconjugated (free) or conjugated fraction. The wet procedure of extracting steroids from urine consists of an initial isolation of lipophilic compounds on a column packed with Amberlite XAD-2 resin, followed by enzymatic hydrolysis with β-glucuronidase from Escherichia coli. After extraction, the hydrolyzed steroids are derivatized to the corresponding trimethylsilyl ethers. The derivatized steroids ere analyzed by gas chromatography/mass spectrometry with selected ion monitoring of their characteristic ions. It takes 12 and 26 min to run GC/MS and edit the raw data for nonconjugated and conjugated fractions respectively. ？ 1990 Oxford University Press. English Analysis of anabolic steroids using gc/ms with selected ion monitoring Article 10.1093/jat/14.2.91 1 Journal of Analytical Toxicology, v.14, no.2, pp.91 - 95 Journal of Analytical Toxicology 14 2 91 95 N scopus 2-s2.0-0025234923 Article anabolic agent doping control unclassified drug article doping drug analysis gas chromatography human mass spectrometry methodology priority journal Anabolic Steroids Doping in Sports Human Mass Fragmentography Substance Abuse Detection Escherichia coli