Kim, Tae-Young Lee, Bum Soo Jo, Beom-Geun Heo, Seong Pil Jung, Young Suk Kim, Su-Nam Kim, Ki Hyun Yang, Min Hye 2024-02-13T05:30:18Z 2024-02-13T05:30:18Z 2024-02-13 2024-01 1661-6596 https://pubs.kist.re.kr/handle/201004/148613 Nymphoides peltata has been used as a medicinal herb in traditional medicines to treat strangury, polyuria, and swelling. The phytochemical investigation of the MeOH extract of N. peltata roots led to the isolation of three iridoid glycosides and three coumarin glycoside derivatives, which were characterized as menthiafolin (1), threoninosecologanin (2), callicoside C (3), and scopolin (4), as well as two undescribed peltatamarins A (5) and B (6). The chemical structures of the undescribed compounds were determined by analyzing their 1 dimensional (D) and 2D nuclear magnetic resonance (NMR) spectra and using high-resolution (HR)-electrospray ionization mass spectroscopy (ESI-MS), along with the chemical reaction of acid hydrolysis. The wound healing activities of the isolated compounds 1-6 were evaluated using a HaCaT cell scratch test. Among the isolates, scopolin (4) and peltatamarin A (5) promoted HaCaT cell migration over scratch wounds, and compound 5 was the most effective. Furthermore, compound 5 significantly promoted cell migration without adversely affecting cell proliferation, even when treated at a high dose (100 mu M). Our results demonstrate that peltatamarin A (5), isolated from N. peltata roots, has the potential for wound healing effects. English Multidisciplinary Digital Publishing Institute (MDPI) Iridoid Glycosides and Coumarin Glycoside Derivatives from the Roots of Nymphoides peltata and Their In Vitro Wound Healing Properties Article 10.3390/ijms25021268 1 International Journal of Molecular Sciences, v.25, no.2 International Journal of Molecular Sciences 25 2 Y scie scopus 001153064300001 2-s2.0-85183385678 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article AQUATIC PLANT Nymphoides peltata peltatamarin A peltatamarin B human keratinocyte cell wound healing cell migration cell proliferation