Kayeong Lim 2024-02-22T08:00:13Z 2024-02-22T08:00:13Z 2024-02-22 2024-01 1976-6696 https://pubs.kist.re.kr/handle/201004/149307 Mitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA nucleases and base editors, including the tools, delivery strategies, and applications. Future advances in mitochondrial genome editing to address challenges regarding their efficiency and specificity can achieve the promise of therapeutic genome editing. English 생화학분자생물학회 Mitochondrial genome editing: strategies, challenges, and applications Article 10.5483/BMBRep.2023-0224 1 BMB Reports, v.57, no.1, pp.19 - 29 BMB Reports 57 1 19 29 Y scie kci ART003047057 001157320400003 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article DNA MUTATIONS GENE-THERAPY MUTANT MTDNA SELECTIVE ELIMINATION BASE EDITORS HETEROPLASMY ENDONUCLEASE DEGRADATION SEQUENCE NUCLEAR Mitochondrial DNA mutations Mitochondrial genome editing mtDNA base editor mtDNA nuclease Protein-based mtDNA editing tools