Kim, Hye Yoon Kang, Bok Eum Lee, Pa Reum Kim, Kyungmin Hong, Gyu-Sang 2024-02-28T00:30:06Z 2024-02-28T00:30:06Z 2024-01-08 2024-04 1863-2653 https://pubs.kist.re.kr/handle/201004/149336 Malformation during cortical development can disrupt the balance of excitatory and inhibitory neural circuits, contributing to various psychiatric and developmental disorders. One of the critical factors of cortical neural networks is the fine regulation of neurogenesis through mechanical cues, such as shear stress and substrate stiffness. Piezo1, a mechanically-activated channel, serves as a transducer for these mechanical cues, regulating embryogenesis. However, specific cell-type expression patterns of this channel during cortical development have not yet been characterized. In the present study, we conducted an RNAscope experiment to visualize the location of Piezo1 transcripts with embryonic neuronal/glial lineage cell markers. Our analysis covered coronal sections of the mouse forebrain on embryonic day 12.5 (E12.5), E14.5, E16.5, and E18.5. In addition, applying Yoda1, a specific Piezo1 agonist, evoked distinct calcium elevation in piriform cortices of E16.5 and E18.5 embryonic slices. Furthermore, pharmacological activation or inhibition of this channel significantly modulated the migration of neurosphere-derived cells in vitro. These findings contribute valuable insights to the field of mechanobiology and provide an understanding of the intricate processes underlying embryonic brain development. English Springer Verlag Expression patterns of Piezo1 in the developing mouse forebrain Article 10.1007/s00429-024-02759-7 1 Brain Structure and Function, v.229, no.3, pp.759 - 773 Brain Structure and Function 229 3 759 773 N scie scopus 001172680200001 Anatomy & Morphology Neurosciences Anatomy & Morphology Neurosciences & Neurology Article ACTIVATED ION-CHANNEL RADIAL GLIA CELLS NEURONS COMPONENTS MIGRATE TBR2 Brain development Embryogenesis Neurogenesis Migration Piezo1 Mechanical cues