Jeon, Eunyoung Koo, Bonhan Kim, Suyeon Kim, Jieun Yu, Yeonuk Jang, Hyowon Lee, Minju Kim, Sung-Han Kang, Taejoon Kim, Sang Kyung Kwak, Rhokyun Shin, Yong Lee, Joonseok 2024-03-21T08:00:23Z 2024-03-21T08:00:23Z 2024-03-21 2024-02 https://pubs.kist.re.kr/handle/201004/149504 Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids. Efficient enrichment and isolation of pathogens are crucial for accurate and sensitive disease identification. Here, the authors present a chip equipped with biporous nanofilms that induces a nanovetex in a microfluidic channel for nucleic acid enrichment, isolation, and detection. English Nature Publishing Group Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection Article 10.1038/s41467-024-45467-w 1 Nature Communications, v.15, no.1 Nature Communications 15 1 Y scie scopus 001162717700022 2-s2.0-85185343972 Multidisciplinary Sciences Science & Technology - Other Topics Article FABRICATION