Park, Yun Ji Choi, Yeong Bin Oh, Sang-Bin Moon, Jinyoung Truong, To Quyen Huynh, Phuong Kim Kim, Sang Min 2024-04-04T02:01:15Z 2024-04-04T02:01:15Z 2024-04-04 2024-03 2468-0834 https://pubs.kist.re.kr/handle/201004/149570 Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC-DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2MODIFIER LETTER PRIME-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract. English 한국응용생명화학회 Development and application of a high-performance liquid chromatography diode-array detection (HPLC-DAD) method for the simultaneous quantification of phenolic compounds in the aerial part of Glehnia littoralis Article 10.1186/s13765-024-00884-9 1 Applied Biological Chemistry, v.67, no.1 Applied Biological Chemistry 67 1 Y scie scopus kci ART003061937 001189364800001 2-s2.0-85188307737 Food Science & Technology Food Science & Technology Article CONSTITUENTS RADIX High-performance liquid chromatography Diode-array detection Phenolic compound Glehnia littoralis Antioxidant activity