Lee, Jae Hoon Lee, Unseok Yoo, Ji Hye Lee, Taek Sung Jung, Je Hyeong Kim, Hyoung Seok 2024-04-04T02:31:29Z 2024-04-04T02:31:29Z 2024-04-04 2024-03 1746-4811 https://pubs.kist.re.kr/handle/201004/149575 Background Plant scientists have largely relied on pathogen growth assays and/or transcript analysis of stress-responsive genes for quantification of disease severity and susceptibility. These methods are destructive to plants, labor-intensive, and time-consuming, thereby limiting their application in real-time, large-scale studies. Image-based plant phenotyping is an alternative approach that enables automated measurement of various symptoms. However, most of the currently available plant image analysis tools require specific hardware platform and vendor specific software packages, and thus, are not suited for researchers who are not primarily focused on plant phenotyping. In this study, we aimed to develop a digital phenotyping tool to enhance the speed, accuracy, and reliability of disease quantification in Arabidopsis. Results Here, we present the Arabidopsis Disease Quantification (AraDQ) image analysis tool for examination of flood-inoculated Arabidopsis seedlings grown on plates containing plant growth media. It is a cross-platform application program with a user-friendly graphical interface that contains highly accurate deep neural networks for object detection and segmentation. The only prerequisite is that the input image should contain a fixed-sized 24-color balance card placed next to the objects of interest on a white background to ensure reliable and reproducible results, regardless of the image acquisition method. The image processing pipeline automatically calculates 10 different colors and morphological parameters for individual seedlings in the given image, and disease-associated phenotypic changes can be easily assessed by comparing plant images captured before and after infection. We conducted two case studies involving bacterial and plant mutants with reduced virulence and disease resistance capabilities, respectively, and thereby demonstrated that AraDQ can capture subtle changes in plant color and morphology with a high level of sensitivity. Conclusions AraDQ offers a simple, fast, and accurate approach for image-based quantification of plant disease symptoms using various parameters. Its fully automated pipeline neither requires prior image processing nor costly hardware setups, allowing easy implementation of the software by researchers interested in digital phenotyping of diseased plants. English BioMed Central AraDQ: an automated digital phenotyping software for quantifying disease symptoms of flood-inoculated Arabidopsis seedlings Article 10.1186/s13007-024-01171-w 1 Plant Methods, v.20, no.1 Plant Methods 20 1 Y scie scopus 001186033000001 2-s2.0-85188066768 Biochemical Research Methods Plant Sciences Biochemistry & Molecular Biology Plant Sciences Article SYRINGAE PV. TOMATO PSEUDOMONAS-SYRINGAE INNATE IMMUNITY HRP PILUS VIRULENCE RECEPTOR GROWTH DC3000 IDENTIFICATION PERCEPTION Arabidopsis Deep learning Digital phenotyping Disease quantification Image analysis Pseudomonas syringae