Jung, Yong Hun Kim, In Kyoung Eom, So Young Kim, Hyunsoo Park, Kyungwon Chung, Seok Lee, Sang Haak Yeo, Chang Dong Kim, Hye Joung Kang, Hye Seon 2024-04-11T04:30:44Z 2024-04-11T04:30:44Z 2024-04-11 2024-10 1738-642X https://pubs.kist.re.kr/handle/201004/149634 BackgroundDespite its low incidence rate, small-cell lung cancer (SCLC), which accounts for approximately 10%-15% of all lung cancers, is concerningly characterized by its rapid progression, strong metastatic potential, and high fatality rate. Therefore, an effective predictive model for SCLC treatment is imperative for early intervention.ObjectiveIn this study, we aimed to enhance the cancer stem cell (CSC) capabilities of SCLC through a three-dimensional (3D) culturing approach and to evaluate its potential as a predictive model for therapeutic response in SCLC by transplanting the spheroids into mice.ResultsTo achieve uniform and scalable production of 3D SCLC spheroids, the SpheroFILM culture platform was employed and the expression of CSC markers was verified at the gene and protein levels within the spheroids cultured onboard. Moreover, spheroids were transplanted into mice to confirm gene and protein expression of CSC markers, tumor-formation capacity, proliferation ability, and invasion potential through histological staining.ConclusionBased on our results, the transplantation of enhanced CSC-functioning 3D SCLC cell lines into mice led to a significant increase in tumor-formation capacity along with elevated expression of CSC markers. Thus, the results collectively suggest that the augmentation of CSC properties in 3D cultured cell lines, when transplanted into animal models, reduces necrosis and enhances tumor formation, proliferation, and invasion. English 대한독성 유전단백체 학회 Establishment of a small-cell lung cancer (SCLC) mouse model using enhanced cancer stem-cell-functioning 3D SCLC spheroids Article 10.1007/s13273-024-00435-9 1 Molecular & Cellular Toxicology, v.20, no.4, pp.1035 - 1043 Molecular & Cellular Toxicology 20 4 1035 1043 N scie scopus kci 001190074900001 2-s2.0-85188160108 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article Small-cell lung cancer Spheroids Cancer stem cells Mouse models Transplantation