Lee Hye Lim Ju-Young Park Jubeen Yoon Wonjin Park Kang, Miso Lee, Jee Hee Gyubin Park Minseob Koh Hyejin Kim Chang Ho Sohn Lee, Sang hee Eunha Kim 2024-04-24T07:40:19Z 2024-04-24T07:40:19Z 2024-04-23 2024-08 https://pubs.kist.re.kr/handle/201004/149710 Stimulator of interferon genes (STING) is an endoplasmic reticulum membrane protein that plays a vital role in innate immunity. In this study, we report a new bioorthogonal chemical probe derived from a previously reported synthetic STING agonist by properly installing a trans-cyclooctene group without perturbation of the efficacy of the ligand. The probe is employed to investigate localization dynamics of STING in live cultured human cells and fixed mouse brain tissues. We showed that an optimized labeling strategy employing the probe and tetrazine-silicon rhodamine fluorophore conjugates in a stepwise manner enables the successful tracking of STING in live cells using fluorescence microscopy. Overall, our bioorthogonal approach using the probe will facilitate in-depth investigations of intricate mechanisms associated with STING. English Elsevier BV Bioorthogonal imaging probe for STING protein in live cell and fixed tissue Article 10.1016/j.snb.2024.135773 1 Sensors and Actuators, B: Chemical, v.412 Sensors and Actuators, B: Chemical 412 N scie scopus 001325408100001 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article Stimulator of interferon Gene Bioorthogonal fluorescent probe Tetrazine ligation Click chemistry Fluorescent imaging probe