Choi, Yoonji Jeong, Jin-Young Hong, Seunghun 2024-04-24T08:02:18Z 2024-04-24T08:02:18Z 2024-02-07 2024-01 1944-8244 https://pubs.kist.re.kr/handle/201004/149723 Adenosine metabolism through adenosine receptors plays a critical role in lung cancer biology. Although recent studies showed the potential of targeting adenosine receptors as drug targets for lung cancer treatment, conventional methods for investigating receptor activities often suffer from various drawbacks, including low sensitivity and slow analysis speed. In this study, adenosine receptor activities in nonsmall cell lung cancer (NSCLC) cells were monitored in real time with high sensitivity through a carbon nanotube field-effect transistor (CNT-FET). In this method, we hybridized a CNT-FET with NSCLC cells expressing A(2A) and A(2B) adenosine receptors to construct a hybrid platform. This platform could detect adenosine, an endogenous ligand of adenosine receptors, down to 1 fM in real time and sensitively discriminate adenosine among other nucleosides. Furthermore, we could also utilize the platform to detect adenosine in complicated environments, such as human serum. Notably, our hybrid platform allowed us to monitor pharmacological effects between adenosine and other drugs, including dipyridamole and theophylline, even in human serum samples. These results indicate that the NSCLC cell-hybridized CNT-FET can be a practical tool for biomedical applications, such as the evaluation and screening of drug-candidate substances. English American Chemical Society Highly Sensitive Real-Time Monitoring of Adenosine Receptor Activities in Nonsmall Cell Lung Cancer Cells Using Carbon Nanotube Field-Effect Transistors Article 10.1021/acsami.3c14492 1 ACS Applied Materials & Interfaces, v.16, no.2, pp.2101 - 2109 ACS Applied Materials & Interfaces 16 2 2101 2109 N scie scopus 001144603700001 2-s2.0-85181822713 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article LABEL-FREE DETECTION TASTE RECEPTOR DIPYRIDAMOLE SPECTROSCOPY FIBRONECTIN MOLECULES APTAMER BINDING CALU-3 nonsmall cell lung cancer cell adenosine receptor adenosine pharmacology carbon nanotube field-effect transistor