Lee, Yong-Jik Jang, Yoo-Na Han, Yoon-Mi Kim, Hyun-Min Seo, Hong Seog Kim, Hyoung Ja Jung, Tae Woo Jeong, Ji Hoon Abd El-Aty, A. M. Jung, Kyung Oh 2024-05-02T03:00:03Z 2024-05-02T03:00:03Z 2024-05-02 2024-06 1096-620X https://pubs.kist.re.kr/handle/201004/149778 To probe the functions of Aster glehni (AG) extract containing various caffeoylquinic acids on dyslipidemia, obesity, and skeletal muscle-related diseases focused on the roles of skeletal muscle, we measured the levels of biomarkers involved in oxidative phosphorylation and type change of skeletal muscle in C2C12 cells and skeletal muscle tissues from apolipoprotein E knockout (ApoE KO) mice. After AG extract treatment in cell and animal experiments, western blotting, immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA) were used to estimate the levels of proteins that participated in skeletal muscle type change and oxidative phosphorylation. AG extract elevated protein expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1 alpha), phosphorylated 5 '-AMP-activated protein kinase (p-AMPK), peroxisome proliferator-activated receptor beta/delta (PPAR beta/delta), myoblast determination protein 1 (MyoD), and myoglobin in skeletal muscle tissues. Furthermore, it elevated the ATP concentration. However, protein expression of myostatin was decreased by AG treatment. In C2C12 cells, increments of MyoD, myoglobin, myosin, ATP-producing pathway, and differentiation degree by AG were dependent on PPAR beta/delta and caffeoylquinic acids. AG extract can contribute to the amelioration of skeletal muscle inactivity and sarcopenia through myogenesis in skeletal muscle tissues from ApoE KO mice, and function of AG extract may be dependent on PPAR beta/delta, and the main functional constituents of AG are trans-5-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid. In addition, in skeletal muscle, AG has potent efficacies against dyslipidemia and obesity through the increase of the type 1 muscle fiber content to produce more ATP by oxidative phosphorylation in skeletal muscle tissues from ApoE KO mice. English 한국식품영양과학회 Aster glehni Extract, Including Caffeoylquinic Acids as the Main Constituents, Induces PPAR β/δ-Dependent Muscle-Type Change and Myogenesis in Apolipoprotein E Knockout Mice Article 10.1089/jmf.2024.k.0027 1 Journal of Medicinal Food, v.27, no.6, pp.521 - 532 Journal of Medicinal Food 27 6 521 532 N scie scopus kci 001206387500001 Chemistry, Medicinal Food Science & Technology Nutrition & Dietetics Pharmacology & Pharmacy Food Science & Technology Nutrition & Dietetics Article OXIDATIVE ENZYME-ACTIVITY HUMAN SKELETAL-MUSCLE FIBER-TYPE MESSENGER-RNA OBESITY EXPRESSION MYOGLOBIN DYSLIPIDEMIA PGC-1-ALPHA METABOLISM sarcopenia obesity PPAR beta/delta Aster glehni myogenesis