Kim, So Yun Guk, Dagyeong Jeong, Youngdo Kim, Eunji Kim, Hansol Kim, Sung Tae 2024-05-09T01:30:05Z 2024-05-09T01:30:05Z 2024-05-09 2024-04 https://pubs.kist.re.kr/handle/201004/149797 Extracellular vesicles play an important role in intercellular communication, with the potential to serve as biomaterials for nanocarriers. Combining such extracellular vesicles and liposomes results in advanced drug delivery carriers. In this study, we attempted to fabricate hybrid vesicles using a membrane fusion method and incorporated an anticancer drug. As a result, we successfully prepared nanosized uniform hybrid vesicles and evaluated their physicochemical characteristics and intracellular uptake mechanisms via endocytosis in various cell lines. Compared to liposomes, the hybrid vesicles showed better physical properties and a relatively higher reduction in cell viability, which was presumably dependent on the specific cell type. These findings suggest that fusion-based hybrid vesicles offer a novel strategy for delivering therapeutic agents and provide insights into the types of extracellular vesicles that are useful in fabricating hybrid vesicles to develop an advanced drug delivery system. English Multidisciplinary Digital Publishing Institute (MDPI) Engineered Hybrid Vesicles and Cellular Internalization in Mammary Cancer Cells Article 10.3390/pharmaceutics16040440 1 Pharmaceutics, v.16, no.4 Pharmaceutics 16 4 Y scie scopus 001209912200001 2-s2.0-85191406945 Pharmacology & Pharmacy Pharmacology & Pharmacy Article EXOSOMES DOXORUBICIN MECHANISMS TOOL extracellular vesicle hybrid vesicle drug delivery intracellular uptake mechanism