Efimov, Andrew I. Hibberd, Timothy J. Wang, Yue Wu, Mingzheng Zhang, Kaiqing Ting, Kaila Madhvapathy, Surabhi Lee, Min-Kyu Kim, Joohee Kang, Jiheon Riahi, Mohammad Zhang, Haohui Travis, Lee Govier, Emily J. Yang, Lianye Kelly, Nigel Huang, Yonggang V？zquez-Guardado, Abraham Spencer, Nick J. Rogers, John A. 2024-05-14T01:00:05Z 2024-05-14T01:00:05Z 2024-05-13 2024-08 0956-5663 https://pubs.kist.re.kr/handle/201004/149825 Wireless activation of the enteric nervous system (ENS) in freely moving animals with implantable optogenetic devices offers a unique and exciting opportunity to selectively control gastrointestinal (GI) transit in vivo, including the gut-brain axis. Programmed delivery of light to targeted locations in the GI-tract, however, poses many challenges not encountered within the central nervous system (CNS). We report here the development of a fully implantable, battery-free wireless device specifically designed for optogenetic control of the GI-tract, capable of generating sufficient light over large areas to robustly activate the ENS, potently inducing colonic motility ex vivo and increased propulsion in vivo. Use in in vivo studies reveals unique stimulation patterns that increase expulsion of colonic content, likely mediated in part by activation of an extrinsic brain-gut motor pathway, via pelvic nerves. This technology overcomes major limitations of conventional wireless optogenetic hardware designed for the CNS, providing targeted control of specific neurochemical classes of neurons in the ENS and brain-gut axis, for direct modulation of GI-transit and associated behaviours in freely moving animals. English Pergamon Press Ltd. Remote optogenetic control of the enteric nervous system and brain-gut axis in freely-behaving mice enabled by a wireless, battery-free optoelectronic device Article 10.1016/j.bios.2024.116298 1 Biosensors and Bioelectronics, v.258 Biosensors and Bioelectronics 258 N scie scopus 001240891800001 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article OPTICAL-PROPERTIES TISSUES COLON Implantable wireless devices Battery-free Optogenetics Enteric nervous system Colon Gut-brain axis