Han, Jun-Hyeok Seo, Hee Seung Lee, Jiyoung Chen, Zheng Wang, Qiyue Lee, Yun Young Lee, Na Kyeong Kang, Jeon Min Kim, Song Hee Hong, Hwichan Park, Jung-Hoon Piao, Yuanzhe Li, Fangyuan Na, Kun Park, Chun Gwon Park, Wooram Ling, Daishun 2024-05-23T02:30:26Z 2024-05-23T02:30:26Z 2024-05-23 2024-05 1385-8947 https://pubs.kist.re.kr/handle/201004/149876 Ferroptosis, an iron -dependent regulated cell death pathway, has emerged as a promising modality for cancer therapy. However, current iron -based ferroptosis inducers, which trigger the Fenton reaction and release Fe2+, face challenges associated with limited cytosolic Fe2+ accumulation, leading to suboptimal ferroptosis induction. Herein, we report an electro-ferroptotic nanoammunition (EFN) composed of iron oxide nanoassembly (IONA) and ascorbic acid -loaded liposomes (Lip -AA) that enables image -guided, spatiotemporally controlled ferroptosis induction via irreversible electroporation (IRE) for enhanced cancer ferroptotic therapy. The IONA and Lip -AA form stable complexes through electrostatic interactions. Upon IRE stimulation, ascorbic acid is released from liposomes and reduce IONA to release abundant Fe2+. Moreover, IRE enhances tumor cell membrane permeability, thus facilitating efficient cytosolic Fe2+ accumulation for effective tumor ferroptosis. Notably, the Fe2+ release of EFN after IRE can be readily monitored by magnetic resonance imaging. Finally, IRE -triggered EFN demonstrates superior tumor growth inhibition, increased survival rates, and activation of immune cells, showing great potential for the development of next -generation spatiotemporally controlled ferroptotic therapies. English Elsevier BV An electro-ferroptotic nanoammunition enables image-guided, spatiotemporally controlled cancer ferroptosis induction via irreversible electroporation Article 10.1016/j.cej.2024.150366 1 Chemical Engineering Journal, v.487 Chemical Engineering Journal 487 N scie scopus 001219357200001 2-s2.0-85188842095 Engineering, Environmental Engineering, Chemical Engineering Article THERMAL ABLATION EFFICACY Irreversible electroporation Ferroptosis Liposomes, Image-guided therapy Cancer immunotherapy