Truong, To Quyen Park, Yun Ji Jeon, Je-Seung Choi, Jaeyoung Koo, Song Yi Choi, Yeong Bin Huynh, Phuong Kim Moon, Jinyoung Kim, Sang Min 2024-05-23T02:30:34Z 2024-05-23T02:30:34Z 2024-05-23 2024-05 1471-2229 https://pubs.kist.re.kr/handle/201004/149878 Background Wasabi, a Brassicaceae member, is well-known for its unique pungent and hot flavor which is produced from glucosinolate (GSL) degradation. Myrosinase (MYR) is a principle enzyme catalyzing the primary conversion of GSLs to GSL hydrolysis products (GHPs) which is responsible for plant defense system and food quality. Due to the limited information in relation to MYRs present in wasabi (Wasabia japonica M.), this study aimed to identify the MYR isogenes in W. japonica and analyze their roles in relation to GSL metabolism. Results In results, WjMYRI-1 was abundantly expressed in all organs, whereas WjMYRI-2 showed only trace expression levels. WjMYRII was highly expressed in the aboveground tissues. Interestingly, WjMYRII expression was significantly upregulated by certain abiotic factors, such as methyl jasmonate (more than 40-fold in petioles and 15-fold in leaves) and salt (tenfold in leaves). Young leaves and roots contained 97.89 and 91.17 mu mol.g(-1) of GSL, whereas less GSL was produced in mature leaves and petioles (38.36 and 44.79 mu mol.g(-1), respectively). Similar pattern was observed in the accumulation of GHPs in various plant organs. Notably, despite the non-significant changes in GSL production, abiotic factors treated samples enhanced significantly GHP content. Pearson's correlation analysis revealed that WjMYRI-1 expression significantly correlated with GSL accumulation and GHP formation, suggesting the primary role of WjMYRI-1-encoding putative protein in GSL degradation. In contrast, WjMYRII expression level showed no correlation with GSL or GHP content, suggesting another physiological role of WjMYRII in stress-induced response. Conclusions In conclusions, three potential isogenes (WjMYRI-1, WjMYRI-2, and WjMYRII) encoding for different MYR isoforms in W. japonica were identified. Our results provided new insights related to MYR and GSL metabolism which are important for the implications of wasabi in agriculture, food and pharmaceutical industry. Particularly, WjMYRI-1 may be primarily responsible for GSL degradation, whereas WjMYRII (clade II) may be involved in other regulatory pathways induced by abiotic factors. English BioMed Central Myrosinase isogenes in wasabi (Wasabia japonica Matsum) and their putative roles in glucosinolate metabolism Article 10.1186/s12870-024-05057-w 1 BMC Plant Biology, v.24, no.1 BMC Plant Biology 24 1 Y scie scopus 001214547500002 2-s2.0-85191737370 Plant Sciences Plant Sciences Article ANTIOXIDANT ACTIVITY EUTREMA-JAPONICUM DIFFERENT ORGANS SALT STRESS INSECT ROOT ACCUMULATION PURIFICATION HYDROLYSIS Glucosinolates Glucosinolate hydrolysis products GSL-MYR defense system Myrosinase Wasabia japonica Abiotic stress Methyl jasmonate