Hyun, Jiyu Eom, Jiin Im, Jisoo Kim, Yu-Jin Seo, Inwoo Kim, Sung -Won Im, Gwang-Bum Kim, Yeong Hwan Lee, Dong-Hyun Park, Hyun Su Yun, Dae Won Kim, Dong-Ik Yoon, Jeong-Kee Um, Soong Ho Yang, Dae Hyeok Bhang, Suk Ho 2024-05-23T05:30:07Z 2024-05-23T05:30:07Z 2024-05-23 2024-04 0168-3659 https://pubs.kist.re.kr/handle/201004/149887 Fibroblasts (hDFs) are widely employed for skin regeneration and the treatment of various skin disorders, yet research were rarely investigated about restoration of diminished therapeutic efficacy due to cell senescence. The application of stem cell and stem cell-derived materials, exosomes, were drawn attention for the restoration functionality of fibroblasts, but still have limitation for unintended side effect or low yield. To advance, stem cellderived nanovesicle (NV) have developed for effective therapeutic reagents with high yield and low risk. In this study, we have developed a method using red light irradiated human adipose-derived stem cells (hADSCs) derived NV (R-NVs) for enhancing the therapeutic efficacy and rejuvenating hDFs. Through red light irradiation, we were able to significantly increase the content of stemness factors and angiogenic biomolecules in R-NVs. Treatment with these R-NVs was found to enhance the migration ability and leading to rejuvenation of old hDFs to levels similar to those of young hDFs. In subsequent in vivo experiments, the treatment of old hDFs with R-NVs demonstrated a superior skin wound healing effect, surpassing that of young hDFs. In summary, this study successfully induced rejuvenation and leading to increased therapeutic efficacy to R-NVs treated old hDFs previously considered as biowaste. English Elsevier BV Fibroblast function recovery through rejuvenation effect of nanovesicles extracted from human adipose-derived stem cells irradiated with red light Article 10.1016/j.jconrel.2024.02.047 1 Journal of Controlled Release, v.368, pp.453 - 465 Journal of Controlled Release 368 453 465 N scie scopus 001218315200001 2-s2.0-85186954824 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article TRANSCRIPTIONAL REGULATION CELLULAR SENESCENCE GROWTH-FACTOR PROLIFERATION PHOTOBIOMODULATION EXPRESSION INVASION TISSUE PLURIPOTENCY RECRUITMENT Aging Fibroblast Fibroblast rejuvenation Human adipose -derived mesenchymal stem cell Photobiomodulation Stem cell therapy Stem cell nanovesicle Wound closing