Choi, Byeong Min Kim, Minkyeong Hong, Hyehyun Park, Tae-Jin Kim, Changmu Park, Jin-Soo Chi, Won-Jae Kim, Seung-Young 2024-05-30T09:00:08Z 2024-05-30T09:00:08Z 2024-05-30 2024-04 1017-7825 https://pubs.kist.re.kr/handle/201004/149956 There has been a growing interest in skin beauty and antimelanogenic products. Melanogenesis is the process of melanin synthesis whereby melanocytes are activated by UV light or hormone stimulation to produce melanin. Melanogenesis is mediated by several enzymes, such as tyrosinase (TYR), microphthalmia-associated transcription factor (MITF), tyrosinase-related protein-1 (TRP-1), and TRP-2. In this study, we investigated the effect of Tuber himalayense extract on melanin synthesis in alpha-melanocyte-stimulating hormone (alpha-MSH)-treated B16F10 melanoma cells. We confirmed that T. himalayense extract was not toxic to alpha-MSH-treated B16F10 melanoma cells and exhibited a significant inhibitory effect on melanin synthesis at concentrations of 25, 50, and 100 mu g/ml. Additionally, the T. himalayense extract inhibited melanin, TRP-1, TRP-2, tyrosinase, and MITF, which are enzymes involved in melanin synthesis, in a concentration-dependent manner. Furthermore, T. himalayense extract inhibited the mitogen-activated protein kinase (MAPK) pathways, such as extracellular signal-regulated kinase-1/2 (ERK), c-Jun N-terminal kinase (JNK), and p38. Therefore, we hypothesized that various components of T. himalayense extract affect multiple factors involved in melanogenesis in B16F10 cells. Our results indicate that T. himalayense extract could potentially be used as a new material for preparing whitening cosmetics. English 한국미생물·생명공학회 Melanin Inhibitory Effect of Tuber himalayense Isolated in Incheon, Korea Article 10.4014/jmb.2311.11021 1 Journal of Microbiology and Biotechnology, v.34, no.4, pp.949 - 957 Journal of Microbiology and Biotechnology 34 4 949 957 Y scie scopus kci ART003077611 001221807400012 2-s2.0-85191615074 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article TRANSCRIPTION FACTOR MELANOGENESIS TARGET CELLS alpha-MSH MAPK Melanogenesis MITF Tuberhimalayense