Song, Euisun Kwon, Jae Won Park, Choul Yong Kang, Jung-Taek Park, Kwideok 2024-06-07T02:00:26Z 2024-06-07T02:00:26Z 2024-06-07 2024-06 https://pubs.kist.re.kr/handle/201004/150013 Human corneal transplantation is still the only option to restore the function of corneal endothelial cells (CECs). Therefore, there is an urgent need for hCEC delivery systems to replace the human donor cornea. Here, we propose an alginate hydrogel (AH)-based delivery system, where a human fibroblast-derived, decellularized extracellular matrix (ECM) was physically integrated with AH. This AH securely combined with the ECM (ECM-AH) was approximately 50 mu m thick, transparent, and permeable. The surface roughness and surface potential provided ECM-AH with a favorable microenvironment for CEC adhesion and growth in vitro. More importantly, ECM-AH could support the structural (ZO-1) and functional (Na+/K+-ATPase) markers of hCECs, as assessed via western blotting and quantitative polymerase chain reaction, which were comparable with those of a ferritic nitrocarburizing (FNC)-coated substrate (a positive control). The cell density per unit area was also significantly better with ECM-AH than the FNC substrate at day 7. A simulation test of cell engraftment in vitro showed that hCECs were successfully transferred into the decellularized porcine corneal tissue, where they were mostly alive. Furthermore, we found out that the endothelial-mesenchymal transition (EnMT)-inductive factors (Smad2 and vimentin) were largely declined with the hCECs grown on ECM-AH, whereas the EnMT inhibitory factor (Smad7) was significantly elevated. The difference was statistically significant compared to that of the FNC substrate. Moreover, we also observed that TGF-beta 1-treated hCECs showed faster recovery of cell phenotype on the ECM. Taken together, our study demonstrates that ECM-AH is a very promising material for hCEC culture and delivery, which endows an excellent microenvironment for cell function and phenotype maintenance. English American Chemical Society Alginate Hydrogel Integrated with a Human Fibroblast-Derived Extracellular Matrix Supports Corneal Endothelial Cell Functionality and Suppresses Endothelial-Mesenchymal Transition Article 10.1021/acsbiomaterials.4c00040 1 ACS Biomaterial Science & Engineering, v.10, no.6, pp.3855 - 3867 ACS Biomaterial Science & Engineering 10 6 3855 3867 N scie scopus 001230241000001 2-s2.0-85194286546 Materials Science, Biomaterials Materials Science Article ADHESION SCAFFOLD GROWTH FILMS REGENERATION PHYSIOLOGY cell-derived extracellularmatrix alginate hydrogel cornea cornealendothelial cells endothelial-mesenchymaltransition (EnMT)