Kim, Seong-Eun Yun, Suji Doh, Junsang Kim, Hong Nam 2024-06-20T05:30:20Z 2024-06-20T05:30:20Z 2024-06-20 2024-10 2192-2640 https://pubs.kist.re.kr/handle/201004/150106 Cancer immunotherapy is used to treat tumors by modulating the immune system. Although the anticancer efficacy of cancer immunotherapy has been evaluated prior to clinical trials, conventional in vivo animal and endpoint models inadequately replicate the intricate process of tumor elimination and reflect human-specific immune systems. Therefore, more sophisticated models that mimic the complex tumor-immune microenvironment must be employed to assess the effectiveness of immunotherapy. Additionally, using real-time imaging technology, a step-by-step evaluation can be applied, allowing for a more precise assessment of treatment efficacy. Here, an overview of the various imaging-based evaluation platforms recently developed for cancer immunotherapeutic applications is presented. Specifically, a fundamental technique is discussed for stably observing immune cell-based tumor cell killing using direct imaging, a microwell that reproduces a confined space for spatial observation, a droplet assay that facilitates cell-cell interactions, and a 3D microphysiological system that reconstructs the vascular environment. Furthermore, it is suggested that future evaluation platforms pursue more human-like immune systems. The imaging-based cancer immunotherapy evaluation platform facilitates systematic assessment of treatments through suitable in vitro models that closely mimic the human immune system. These evaluation methods enable a more precise and personalized approach in the development of cancer immunotherapy. image English Wiley-Blackwell Imaging-Based Efficacy Evaluation of Cancer Immunotherapy in Engineered Tumor Platforms and Tumor Organoids Article 10.1002/adhm.202400475 1 Advanced Healthcare Materials, v.13, no.27 Advanced Healthcare Materials 13 27 Y scie scopus 001240518300001 2-s2.0-85195284842 Engineering, Biomedical Nanoscience & Nanotechnology Materials Science, Biomaterials Engineering Science & Technology - Other Topics Materials Science Review PROSTATE-CANCER RELEASE-ASSAY NATURAL-KILLER-CELLS T-CELLS CYTOTOXICITY ASSAY IMMUNE CELLS IN-VITRO ANNEXIN-V MIGRATION BEHAVIOR SPHEROID CULTURE bioimaging cancer immunotherapy efficacy evaluation engineered tumor platform tumor organoid