Jeong, Daun Jang, Seyoun Roh, Soonjong Choi, Ji Hye Seo, I Ji Lee, Jin Hyuck Kim, Ji Hoon Kwon, Ilkeun Jung, Youngmee Hwang, Jangsun Jang, Woo Young Yoo, Jin 2024-06-26T09:30:09Z 2024-06-26T09:30:09Z 2024-06-26 2024-08 1385-8947 https://pubs.kist.re.kr/handle/201004/150119 Sprayable hydrogels offer a promising approach for treating diabetic wounds. They possess the capability to conform to irregular surfaces, create a moist environment that supports the growth of new tissue, and offer protection against infections. Yet, applying previously reported sprayable hydrogels has proven to be challenging, often necessitating the use of specialized equipment or the simultaneous injection of multiple components. In this study, we develop a user-friendly therapeutic hydrogel that can be applied using a general spray bottle. This hydrogel is formulated using the bioactive agent, LL37, which is known for its antimicrobial properties and ability to enhance angiogenesis. Additionally, optical mRNA nanosensors, NanoFlares, were incorporated into the hydrogel to measure the expression of proliferation and inflammation biomarkers, providing precise molecular-based parameters for evaluating diabetic wound status. The efficacy of LL37- and NF-incorporated hydrogels in promoting in vivo wound healing in normal and diabetic mice is evaluated, and a Wound Healing Index (WHI) is presented to assist medical professionals in making informed decisions about wound care and treatment. Overall, our study demonstrates the potential of sprayable hydrogels for promoting diabetic wound healing and monitoring the diabetic wound status, which could have significant potential for future clinical applications. English Elsevier BV Sprayable hydrogel with optical mRNA nanosensors for Real-Time monitoring and healing of diabetic wounds Article 10.1016/j.cej.2024.152711 1 Chemical Engineering Journal, v.493 Chemical Engineering Journal 493 N scie scopus 001251416900001 Engineering, Environmental Engineering, Chemical Engineering Article INFLAMMATION ADHESION PECAM-1 CLAY DRESSINGS LAPONITE Sprayable hydrogels Nanosensor Wound healing Diabetic wound Antimicrobial peptides