Cho, Hee Young Lee, Ji Eun Park, Kyo Hoon Choi, Bo Young Lee, Min Jung Jeong, Da Eun Shin, Sue 2024-06-28T02:00:09Z 2024-06-28T02:00:09Z 2024-06-27 2024-06 https://pubs.kist.re.kr/handle/201004/150121 This study aimed to identify plasma proteins that could serve as potential biomarkers for microbial invasion of the amniotic cavity (MIAC) or intra-amniotic inflammation (IAI) in women with preterm labor (PTL). A retrospective cohort comprised singleton pregnant women with PTL (24？34 weeks) who underwent amniocentesis. Pooled plasma samples were analyzed by label-free liquid chromatography-tandem mass spectrometry for proteome profiling in a nested case？control study (concomitant MIAC/IAI cases vs. non-MIAC/IAI controls [n？=？10 per group]). Eight target proteins associated with MIAC/IAI were further verified by immunoassays in a large cohort (n？=？230). Shotgun proteomic analysis revealed 133 differentially expressed proteins (fold change？>？1.5, P？<？0.05) in the plasma of MIAC/IAI cases. Further quantification confirmed that the levels of AFP were higher and those of kallistatin and TGFBI were lower in the plasma of women with MIAC and that the levels of kallistatin and TGFBI were lower in the plasma of women with IAI than in those without these conditions. The area under the curves of plasma AFP, kallistatin, and TGFBI ranged within 0.67？0.81 with respect to each endpoint. In summary, plasma AFP, kallistatin, and TGFBI may represent valuable non-invasive biomarkers for predicting MIAC or IAI in women with PTL. English Nature Publishing Group Identification and characterization of plasma proteins associated with intra-amniotic inflammation and/or infection in women with preterm labor Article 10.1038/s41598-024-65616-x 1 Scientific Reports, v.14, no.1 Scientific Reports 14 1 Y scie scopus 001255006800069 Multidisciplinary Sciences Science & Technology - Other Topics Article SERUM ALPHA-FETOPROTEIN PROTEOMIC ANALYSIS MATERNAL PLASMA BIOMARKERS EXPRESSION PREDICTION EPIDEMIOLOGY PREECLAMPSIA KALLISTATIN AMNIOTIC-FLUID